TY - JOUR
T1 - Multi-laboratory Validation of a New Marine Biodegradation Screening Test for Chemical Persistence Assessment
AU - Ott, Amelie
AU - Martin, Timothy J.
AU - Acharya, Kishor
AU - Lyon, Delina Y.
AU - Robinson, Nik
AU - Rowles, Bob
AU - Snape, Jason R.
AU - Still, Ian
AU - Whale, Graham F.
AU - Albright, Vurtice C.
AU - Bäverbäck, Petra
AU - Best, Nicola
AU - Commander, Ruth
AU - Eickhoff, Curtis
AU - Finn, Sarah
AU - Hidding, Björn
AU - Maischak, Heiko
AU - Sowders, Katherine A.
AU - Taruki, Masanori
AU - Walton, Helen E.
AU - Wennberg, Aina Charlotte
AU - Davenport, Russell J.
N1 - Publisher Copyright:
© 2020 American Chemical Society.
Funding Information:
This work was primarily funded by the European Chemical Industry Council (Cefic) Long-Range Initiative (LRI; project ECO11.2-3). Additional funding support was provided by the Engineering and Physical Sciences Research Council [EPSRC] (grant no.: EP/1025782/1, Challenging Engineering Award).
PY - 2020/4/7
Y1 - 2020/4/7
N2 - Current biodegradation screening tests are not specifically designed for persistence assessment of chemicals, often show high inter- and intra-test variability, and often give false negative biodegradation results. Based on previous studies and recommendations, an international ring test involving 13 laboratories validated a new test method for marine biodegradation with a focus on improving the reliability of screening to determine the environmental degradation potential of chemicals. The new method incorporated increased bacterial cell concentrations to better represent the microbial diversity; a chemical is likely to be exposed in the sampled environments and ran beyond 60 days, which is the half-life threshold for chemical persistence in the marine environment. The new test provided a more reliable and less variable characterization of the biodegradation behavior of five reference chemicals (sodium benzoate, triethanolamine, 4-nitrophenol, anionic polyacrylamide, and pentachlorophenol), with respect to REACH and OSPAR persistence thresholds, than the current OECD 306 test. The proposed new method provides a cost-effective screening test for non-persistence that could streamline chemical regulation and reduce the cost and animal welfare implications of further higher tier testing.
AB - Current biodegradation screening tests are not specifically designed for persistence assessment of chemicals, often show high inter- and intra-test variability, and often give false negative biodegradation results. Based on previous studies and recommendations, an international ring test involving 13 laboratories validated a new test method for marine biodegradation with a focus on improving the reliability of screening to determine the environmental degradation potential of chemicals. The new method incorporated increased bacterial cell concentrations to better represent the microbial diversity; a chemical is likely to be exposed in the sampled environments and ran beyond 60 days, which is the half-life threshold for chemical persistence in the marine environment. The new test provided a more reliable and less variable characterization of the biodegradation behavior of five reference chemicals (sodium benzoate, triethanolamine, 4-nitrophenol, anionic polyacrylamide, and pentachlorophenol), with respect to REACH and OSPAR persistence thresholds, than the current OECD 306 test. The proposed new method provides a cost-effective screening test for non-persistence that could streamline chemical regulation and reduce the cost and animal welfare implications of further higher tier testing.
UR - http://www.scopus.com/inward/record.url?scp=85083003211&partnerID=8YFLogxK
U2 - 10.1021/acs.est.9b07710
DO - 10.1021/acs.est.9b07710
M3 - Article
C2 - 32162906
AN - SCOPUS:85083003211
SN - 0013-936X
VL - 54
SP - 4210
EP - 4220
JO - Environmental Science and Technology
JF - Environmental Science and Technology
IS - 7
ER -