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Multi-compound electrophoretic assays for tyramine oxidase with a UV area detector imaging multiple windows on a looped capillary

Research output: Contribution to journalArticle

Author(s)

  • Pawel L. Urban
  • David M. Goodall
  • Alexandre Z. Carvalho
  • Edmund T. Bergstrom
  • Ann Van Schepdael
  • Neil C. Bruce

Department/unit(s)

Publication details

JournalJournal of Chromatography A
DatePublished - 3 Oct 2008
Issue number1
Volume1206
Number of pages12
Pages (from-to)52-63
Original languageEnglish

Abstract

Active pixel sensor UV area imaging and capacitively coupled contactless conductivity detection have been applied in an electrophoretically mediated microanalysis (EMMA) assay for substrate specificity of tyramine oxidase (Arthrobacter sp.). Use of the UV area imaging detector to monitor four windows in a capillary with three loops provided intrinsic self-referencing for all species and identified tyramine and 2-phenethylamine as the only reactive components in a multi-compound mixture. Continuous engagement EMMA experiments showed significant benefits by comparison with plug-plug EMMA, improving sensitivity by extending enzyme-substrate interaction times and allowing measurement of time-dependent reaction in the substrate zones passing the four windows. (C) 2008 Elsevier B.V. All rights reserved.

    Research areas

  • Biocatalysis, Capillary electrophoresis, On-capillary enzyme assay, Substrate specificity, Tyramine oxidase, UV area imaging, CONTACTLESS CONDUCTIVITY DETECTION, LINEAR PHOTODIODE-ARRAY, PLASMA AMINE OXIDASE, ACTIVE PIXEL SENSOR, MEDIATED MICROANALYSIS, SUBSTRATE-SPECIFICITY, ZONE-ELECTROPHORESIS, MICROCHIP ELECTROPHORESIS, FUNDAMENTAL-ASPECTS, COLORIMETRIC ASSAY

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