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Multi-host environments select for host-generalist conjugative plasmids

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Multi-host environments select for host-generalist conjugative plasmids. / Kottara, Anastasia; Hall, James P J; Harrison, Ellie; Brockhurst, Michael A.

In: Bmc evolutionary biology, Vol. 16, No. 70, 02.04.2016.

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Harvard

Kottara, A, Hall, JPJ, Harrison, E & Brockhurst, MA 2016, 'Multi-host environments select for host-generalist conjugative plasmids', Bmc evolutionary biology, vol. 16, no. 70. https://doi.org/10.1186/s12862-016-0642-z

APA

Kottara, A., Hall, J. P. J., Harrison, E., & Brockhurst, M. A. (2016). Multi-host environments select for host-generalist conjugative plasmids. Bmc evolutionary biology, 16(70). https://doi.org/10.1186/s12862-016-0642-z

Vancouver

Kottara A, Hall JPJ, Harrison E, Brockhurst MA. Multi-host environments select for host-generalist conjugative plasmids. Bmc evolutionary biology. 2016 Apr 2;16(70). https://doi.org/10.1186/s12862-016-0642-z

Author

Kottara, Anastasia ; Hall, James P J ; Harrison, Ellie ; Brockhurst, Michael A. / Multi-host environments select for host-generalist conjugative plasmids. In: Bmc evolutionary biology. 2016 ; Vol. 16, No. 70.

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@article{e892a6a9da814d74aa4d47086bdaa7a4,
title = "Multi-host environments select for host-generalist conjugative plasmids",
abstract = "BACKGROUND: Conjugative plasmids play an important role in bacterial evolution by transferring ecologically important genes within and between species. A key limit on interspecific horizontal gene transfer is plasmid host range. Here, we experimentally test the effect of single and multi-host environments on the host-range evolution of a large conjugative mercury resistance plasmid, pQBR57. Specifically, pQBR57 was conjugated between strains of a single host species, either P. fluorescens or P. putida, or alternating between P. fluorescens and P. putida. Crucially, the bacterial hosts were not permitted to evolve allowing us to observe plasmid evolutionary responses in isolation.RESULTS: In all treatments plasmids evolved higher conjugation rates over time. Plasmids evolved in single-host environments adapted to their host bacterial species becoming less costly, but in the case of P. fluorescens-adapted plasmids, became costlier in P. putida, suggesting an evolutionary trade-off. When evolved in the multi-host environment plasmids adapted to P. fluorescens without a higher cost in P. putida.CONCLUSION: Whereas evolution in a single-host environment selected for host-specialist plasmids due to a fitness trade-off, this trade-off could be circumvented in the multi-host environment, leading to the evolution of host-generalist plasmids.",
author = "Anastasia Kottara and Hall, {James P J} and Ellie Harrison and Brockhurst, {Michael A}",
note = "{\circledC} Kottara et al. 2016",
year = "2016",
month = "4",
day = "2",
doi = "10.1186/s12862-016-0642-z",
language = "English",
volume = "16",
journal = "Bmc evolutionary biology",
issn = "1471-2148",
publisher = "BioMed Central",
number = "70",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Multi-host environments select for host-generalist conjugative plasmids

AU - Kottara, Anastasia

AU - Hall, James P J

AU - Harrison, Ellie

AU - Brockhurst, Michael A

N1 - © Kottara et al. 2016

PY - 2016/4/2

Y1 - 2016/4/2

N2 - BACKGROUND: Conjugative plasmids play an important role in bacterial evolution by transferring ecologically important genes within and between species. A key limit on interspecific horizontal gene transfer is plasmid host range. Here, we experimentally test the effect of single and multi-host environments on the host-range evolution of a large conjugative mercury resistance plasmid, pQBR57. Specifically, pQBR57 was conjugated between strains of a single host species, either P. fluorescens or P. putida, or alternating between P. fluorescens and P. putida. Crucially, the bacterial hosts were not permitted to evolve allowing us to observe plasmid evolutionary responses in isolation.RESULTS: In all treatments plasmids evolved higher conjugation rates over time. Plasmids evolved in single-host environments adapted to their host bacterial species becoming less costly, but in the case of P. fluorescens-adapted plasmids, became costlier in P. putida, suggesting an evolutionary trade-off. When evolved in the multi-host environment plasmids adapted to P. fluorescens without a higher cost in P. putida.CONCLUSION: Whereas evolution in a single-host environment selected for host-specialist plasmids due to a fitness trade-off, this trade-off could be circumvented in the multi-host environment, leading to the evolution of host-generalist plasmids.

AB - BACKGROUND: Conjugative plasmids play an important role in bacterial evolution by transferring ecologically important genes within and between species. A key limit on interspecific horizontal gene transfer is plasmid host range. Here, we experimentally test the effect of single and multi-host environments on the host-range evolution of a large conjugative mercury resistance plasmid, pQBR57. Specifically, pQBR57 was conjugated between strains of a single host species, either P. fluorescens or P. putida, or alternating between P. fluorescens and P. putida. Crucially, the bacterial hosts were not permitted to evolve allowing us to observe plasmid evolutionary responses in isolation.RESULTS: In all treatments plasmids evolved higher conjugation rates over time. Plasmids evolved in single-host environments adapted to their host bacterial species becoming less costly, but in the case of P. fluorescens-adapted plasmids, became costlier in P. putida, suggesting an evolutionary trade-off. When evolved in the multi-host environment plasmids adapted to P. fluorescens without a higher cost in P. putida.CONCLUSION: Whereas evolution in a single-host environment selected for host-specialist plasmids due to a fitness trade-off, this trade-off could be circumvented in the multi-host environment, leading to the evolution of host-generalist plasmids.

U2 - 10.1186/s12862-016-0642-z

DO - 10.1186/s12862-016-0642-z

M3 - Article

VL - 16

JO - Bmc evolutionary biology

JF - Bmc evolutionary biology

SN - 1471-2148

IS - 70

ER -