Multiparameter antibiotic resistance detection based on hydrodynamic trapping of individual E. coli

TY - JOUR

T1 - Multiparameter antibiotic resistance detection based on hydrodynamic trapping of individual E. coli

AU - Pitruzzello, Giampaolo

AU - Thorpe, Stephen

AU - Johnson, Steven

AU - Evans, Adrian

AU - Gadêlha, Hermes

AU - Krauss, Thomas F.

N1 - © The Royal Society of Chemistry 2019.

PY - 2019/3/14

Y1 - 2019/3/14

N2 - There is an urgent need to develop novel methods for assessing the response of bacteria to antibiotics in a timely manner. Antibiotics are traditionally assessed via their effect on bacteria in a culture medium, which takes 24-48 h and exploits only a single parameter, i.e. growth. Here, we present a multiparameter approach at the single-cell level that takes approximately an hour from spiking the culture to correctly classify susceptible and resistant strains. By hydrodynamically trapping hundreds of bacteria, we simultaneously monitor the evolution of motility and morphology of individual bacteria upon drug administration. We show how this combined detection method provides insights into the activity of antimicrobials at the onset of their action which single parameter and traditional tests cannot offer. Our observations complement the current growth-based methods and highlight the need for future antimicrobial susceptibility tests to take multiple parameters into account.

AB - There is an urgent need to develop novel methods for assessing the response of bacteria to antibiotics in a timely manner. Antibiotics are traditionally assessed via their effect on bacteria in a culture medium, which takes 24-48 h and exploits only a single parameter, i.e. growth. Here, we present a multiparameter approach at the single-cell level that takes approximately an hour from spiking the culture to correctly classify susceptible and resistant strains. By hydrodynamically trapping hundreds of bacteria, we simultaneously monitor the evolution of motility and morphology of individual bacteria upon drug administration. We show how this combined detection method provides insights into the activity of antimicrobials at the onset of their action which single parameter and traditional tests cannot offer. Our observations complement the current growth-based methods and highlight the need for future antimicrobial susceptibility tests to take multiple parameters into account.

UR - http://www.scopus.com/inward/record.url?scp=85064179289&partnerID=8YFLogxK

U2 - 10.1039/c8lc01397g

DO - 10.1039/c8lc01397g

M3 - Article

C2 - 30869093

AN - SCOPUS:85064179289

SN - 1473-0197

VL - 19

SP - 1417

EP - 1426

JO - Lab on a chip

JF - Lab on a chip

IS - 8

ER -