mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes

Jessica B A Sadler, Jennifer Roccisana, Minttu Virolainen, Nia J. Bryant, Gwyn W. Gould*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Insulin stimulates the delivery of glucose transporter-4 (GLUT4)-containing vesicles to the surface of adipocytes. Depletion of the Sec1/Munc18 protein mVps45 significantly abrogates insulin-stimulated glucose transport and GLUT4 translocation. Here we show that depletion of mVps45 selectively reduced expression of VAMPs 2 and 4, but not other VAMP isoforms. Although we did not observe direct interaction of mVps45 with any VAMP isoform; we found that the cognate binding partner of mVps45, Syntaxin 16 associates with VAMPs 2, 4, 7 and 8 in vitro. Co-immunoprecipitation experiments in 3T3-L1 adipocytes revealed an interaction between Syntaxin 16 and only VAMP4. We suggest GLUT4 trafficking is controlled by the coordinated expression of mVps45/Syntaxin 16/VAMP4, and that depletion of mVps45 regulates VAMP2 levels indirectly, perhaps via reduced trafficking into specialized subcellular compartments.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalCommunicative & integrative biology
Issue number3
Publication statusPublished - 24 Jun 2015


  • Adipocyte
  • Biochemistry
  • Cell biology
  • Glucose transport
  • Hormones
  • Insulin
  • Intracellular membranes
  • Membrane protein trafficking
  • Membrane trafficking
  • SNARE proteins
  • VAMP

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