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mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes

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mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes. / Sadler, Jessica B A; Roccisana, Jennifer; Virolainen, Minttu; Bryant, Nia J.; Gould, Gwyn W.

In: Communicative & integrative biology, Vol. 8, No. 3, 24.06.2015, p. 1-5.

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Sadler, JBA, Roccisana, J, Virolainen, M, Bryant, NJ & Gould, GW 2015, 'mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes', Communicative & integrative biology, vol. 8, no. 3, pp. 1-5. https://doi.org/10.1080/19420889.2015.1026494

APA

Sadler, J. B. A., Roccisana, J., Virolainen, M., Bryant, N. J., & Gould, G. W. (2015). mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes. Communicative & integrative biology, 8(3), 1-5. https://doi.org/10.1080/19420889.2015.1026494

Vancouver

Sadler JBA, Roccisana J, Virolainen M, Bryant NJ, Gould GW. mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes. Communicative & integrative biology. 2015 Jun 24;8(3):1-5. https://doi.org/10.1080/19420889.2015.1026494

Author

Sadler, Jessica B A ; Roccisana, Jennifer ; Virolainen, Minttu ; Bryant, Nia J. ; Gould, Gwyn W. / mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes. In: Communicative & integrative biology. 2015 ; Vol. 8, No. 3. pp. 1-5.

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@article{090626bdab654490a330c6058c061a9c,
title = "mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes",
abstract = "Insulin stimulates the delivery of glucose transporter-4 (GLUT4)-containing vesicles to the surface of adipocytes. Depletion of the Sec1/Munc18 protein mVps45 significantly abrogates insulin-stimulated glucose transport and GLUT4 translocation. Here we show that depletion of mVps45 selectively reduced expression of VAMPs 2 and 4, but not other VAMP isoforms. Although we did not observe direct interaction of mVps45 with any VAMP isoform; we found that the cognate binding partner of mVps45, Syntaxin 16 associates with VAMPs 2, 4, 7 and 8 in vitro. Co-immunoprecipitation experiments in 3T3-L1 adipocytes revealed an interaction between Syntaxin 16 and only VAMP4. We suggest GLUT4 trafficking is controlled by the coordinated expression of mVps45/Syntaxin 16/VAMP4, and that depletion of mVps45 regulates VAMP2 levels indirectly, perhaps via reduced trafficking into specialized subcellular compartments.",
keywords = "Adipocyte, Biochemistry, Cell biology, Glucose transport, Hormones, Insulin, Intracellular membranes, Membrane protein trafficking, Membrane trafficking, SNARE proteins, VAMP",
author = "Sadler, {Jessica B A} and Jennifer Roccisana and Minttu Virolainen and Bryant, {Nia J.} and Gould, {Gwyn W.}",
year = "2015",
month = "6",
day = "24",
doi = "10.1080/19420889.2015.1026494",
language = "English",
volume = "8",
pages = "1--5",
journal = "Communicative & integrative biology",
issn = "1942-0889",
publisher = "Landes Bioscience",
number = "3",

}

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TY - JOUR

T1 - mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes

AU - Sadler, Jessica B A

AU - Roccisana, Jennifer

AU - Virolainen, Minttu

AU - Bryant, Nia J.

AU - Gould, Gwyn W.

PY - 2015/6/24

Y1 - 2015/6/24

N2 - Insulin stimulates the delivery of glucose transporter-4 (GLUT4)-containing vesicles to the surface of adipocytes. Depletion of the Sec1/Munc18 protein mVps45 significantly abrogates insulin-stimulated glucose transport and GLUT4 translocation. Here we show that depletion of mVps45 selectively reduced expression of VAMPs 2 and 4, but not other VAMP isoforms. Although we did not observe direct interaction of mVps45 with any VAMP isoform; we found that the cognate binding partner of mVps45, Syntaxin 16 associates with VAMPs 2, 4, 7 and 8 in vitro. Co-immunoprecipitation experiments in 3T3-L1 adipocytes revealed an interaction between Syntaxin 16 and only VAMP4. We suggest GLUT4 trafficking is controlled by the coordinated expression of mVps45/Syntaxin 16/VAMP4, and that depletion of mVps45 regulates VAMP2 levels indirectly, perhaps via reduced trafficking into specialized subcellular compartments.

AB - Insulin stimulates the delivery of glucose transporter-4 (GLUT4)-containing vesicles to the surface of adipocytes. Depletion of the Sec1/Munc18 protein mVps45 significantly abrogates insulin-stimulated glucose transport and GLUT4 translocation. Here we show that depletion of mVps45 selectively reduced expression of VAMPs 2 and 4, but not other VAMP isoforms. Although we did not observe direct interaction of mVps45 with any VAMP isoform; we found that the cognate binding partner of mVps45, Syntaxin 16 associates with VAMPs 2, 4, 7 and 8 in vitro. Co-immunoprecipitation experiments in 3T3-L1 adipocytes revealed an interaction between Syntaxin 16 and only VAMP4. We suggest GLUT4 trafficking is controlled by the coordinated expression of mVps45/Syntaxin 16/VAMP4, and that depletion of mVps45 regulates VAMP2 levels indirectly, perhaps via reduced trafficking into specialized subcellular compartments.

KW - Adipocyte

KW - Biochemistry

KW - Cell biology

KW - Glucose transport

KW - Hormones

KW - Insulin

KW - Intracellular membranes

KW - Membrane protein trafficking

KW - Membrane trafficking

KW - SNARE proteins

KW - VAMP

UR - http://www.scopus.com/inward/record.url?scp=84921867303&partnerID=8YFLogxK

U2 - 10.1080/19420889.2015.1026494

DO - 10.1080/19420889.2015.1026494

M3 - Article

VL - 8

SP - 1

EP - 5

JO - Communicative & integrative biology

JF - Communicative & integrative biology

SN - 1942-0889

IS - 3

ER -