By the same authors

NADPH oxidases in Eukaryotes: red algae provide new hints!

Research output: Contribution to journalArticle

Published copy (DOI)

Author(s)

  • Cécile Hervé
  • Thierry Tonon
  • Jonas Collén
  • Erwan Corre
  • Catherine Boyen

Department/unit(s)

Publication details

JournalCurrent genetics
DatePublished - Mar 2006
Issue number3
Volume49
Number of pages15
Pages (from-to)190-204
Original languageEnglish

Abstract

The red macro-alga Chondrus crispus is known to produce superoxide radicals in response to cell-free extracts of its green algal pathogenic endophyte Acrochaete operculata. So far, no enzymes involved in this metabolism have been isolated from red algae. We report here the isolation of a gene encoding a homologue of the respiratory burst oxidase gp91(phox) in C. crispus, named Ccrboh. This single copy gene encodes a polypeptide of 825 amino acids. Search performed in available genome and EST algal databases identified sequences showing common features of NADPH oxidases in other algae such as the red unicellular Cyanidioschyzon merolae, the economically valuable red macro-alga Porphyra yezoensis and the two diatoms Phaeodactylum tricornutum and Thalassiosira pseudonana. Domain organization and phylogenetic relationships with plant, animal, fungal and algal NADPH oxidase homologues were analyzed. Transcription analysis of the C. crispus gene revealed that it was over-transcribed during infection of C. crispus gametophyte by the endophyte A. operculata, and after incubation in presence of atrazine, methyl jasmonate and hydroxyperoxides derived from C20 polyunsaturated fatty acids (PUFAs). These results also illustrate the interest of exploring the red algal lineage for gaining insight into the deep evolution of NADPH oxidases in Eukaryotes.

    Research areas

  • Algal Proteins/genetics, Amino Acid Sequence, Chondrus/enzymology, Cloning, Molecular, Diatoms/chemistry, Eukaryotic Cells/enzymology, Evolution, Molecular, Molecular Sequence Data, NADPH Oxidases/genetics, Phylogeny, Sequence Homology, Amino Acid

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