Natural variation reveals that intracellular distribution of ELF3 protein is associated with function in the circadian clock

M Usman Anwer; Eleni Boikoglou; Eva Herrero; Marc Hallstein; Amanda M Davis; Geo Velikkakam James; Ferenc Nagy; Seth J Davis

doi:10.7554/eLife.02206

Natural variation reveals that intracellular distribution of ELF3 protein is associated with function in the circadian clock

M Usman Anwer, Eleni Boikoglou, Eva Herrero, Marc Hallstein, Amanda M Davis, Geo Velikkakam James, Ferenc Nagy, Seth J Davis

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Natural selection of variants within the Arabidopsis thaliana circadian clock can be attributed to adaptation to varying environments. To define a basis for such variation, we examined clock speed in a reporter-modified Bay-0 x Shakdara recombinant inbred line and localized heritable variation. Extensive variation led us to identify EARLY FLOWERING3 (ELF3) as a major quantitative trait locus (QTL). The causal nucleotide polymorphism caused a short-period phenotype under light and severely dampened rhythm generation in darkness, and entrainment alterations resulted. We found that ELF3-Sha protein failed to properly localize to the nucleus, and its ability to accumulate in darkness was compromised. Evidence was provided that the ELF3-Sha allele originated in Central Asia. Collectively we showed that ELF3 protein plays a vital role in defining its light-repressor action in the circadian clock and that its functional abilities are largely dependent on its cellular localization.

Original language	English
Article number	e02206
Pages (from-to)	e02206
Journal	eLife
Volume	2014
Issue number	3
DOIs	https://doi.org/10.7554/eLife.02206
Publication status	Published - 27 May 2014

Bibliographical note

Access to Document

10.7554/eLife.02206

Cite this

@article{7cb713c3a1d140868f7ed09cfc3378ae,

title = "Natural variation reveals that intracellular distribution of ELF3 protein is associated with function in the circadian clock",

abstract = "Natural selection of variants within the Arabidopsis thaliana circadian clock can be attributed to adaptation to varying environments. To define a basis for such variation, we examined clock speed in a reporter-modified Bay-0 x Shakdara recombinant inbred line and localized heritable variation. Extensive variation led us to identify EARLY FLOWERING3 (ELF3) as a major quantitative trait locus (QTL). The causal nucleotide polymorphism caused a short-period phenotype under light and severely dampened rhythm generation in darkness, and entrainment alterations resulted. We found that ELF3-Sha protein failed to properly localize to the nucleus, and its ability to accumulate in darkness was compromised. Evidence was provided that the ELF3-Sha allele originated in Central Asia. Collectively we showed that ELF3 protein plays a vital role in defining its light-repressor action in the circadian clock and that its functional abilities are largely dependent on its cellular localization.",

author = "Anwer, {M Usman} and Eleni Boikoglou and Eva Herrero and Marc Hallstein and Davis, {Amanda M} and {Velikkakam James}, Geo and Ferenc Nagy and Davis, {Seth J}",

note = "Copyright {\textcopyright} 2014, Anwer et al.",

year = "2014",

month = may,

day = "27",

doi = "10.7554/eLife.02206",

language = "English",

volume = "2014",

pages = "e02206",

journal = "eLife",

issn = "2050-084X",

publisher = "eLife Sciences Publications",

number = "3",

}

TY - JOUR

T1 - Natural variation reveals that intracellular distribution of ELF3 protein is associated with function in the circadian clock

AU - Anwer, M Usman

AU - Boikoglou, Eleni

AU - Herrero, Eva

AU - Hallstein, Marc

AU - Davis, Amanda M

AU - Velikkakam James, Geo

AU - Nagy, Ferenc

AU - Davis, Seth J

PY - 2014/5/27

Y1 - 2014/5/27

N2 - Natural selection of variants within the Arabidopsis thaliana circadian clock can be attributed to adaptation to varying environments. To define a basis for such variation, we examined clock speed in a reporter-modified Bay-0 x Shakdara recombinant inbred line and localized heritable variation. Extensive variation led us to identify EARLY FLOWERING3 (ELF3) as a major quantitative trait locus (QTL). The causal nucleotide polymorphism caused a short-period phenotype under light and severely dampened rhythm generation in darkness, and entrainment alterations resulted. We found that ELF3-Sha protein failed to properly localize to the nucleus, and its ability to accumulate in darkness was compromised. Evidence was provided that the ELF3-Sha allele originated in Central Asia. Collectively we showed that ELF3 protein plays a vital role in defining its light-repressor action in the circadian clock and that its functional abilities are largely dependent on its cellular localization.

AB - Natural selection of variants within the Arabidopsis thaliana circadian clock can be attributed to adaptation to varying environments. To define a basis for such variation, we examined clock speed in a reporter-modified Bay-0 x Shakdara recombinant inbred line and localized heritable variation. Extensive variation led us to identify EARLY FLOWERING3 (ELF3) as a major quantitative trait locus (QTL). The causal nucleotide polymorphism caused a short-period phenotype under light and severely dampened rhythm generation in darkness, and entrainment alterations resulted. We found that ELF3-Sha protein failed to properly localize to the nucleus, and its ability to accumulate in darkness was compromised. Evidence was provided that the ELF3-Sha allele originated in Central Asia. Collectively we showed that ELF3 protein plays a vital role in defining its light-repressor action in the circadian clock and that its functional abilities are largely dependent on its cellular localization.

U2 - 10.7554/eLife.02206

DO - 10.7554/eLife.02206

M3 - Article

C2 - 24867215

SN - 2050-084X

VL - 2014

SP - e02206

JO - eLife

JF - eLife

IS - 3

M1 - e02206

ER -