New Applications for Phage Integrases

TY - JOUR

T1 - New Applications for Phage Integrases

AU - Fogg, Paul C M

AU - Colloms, Sean

AU - Rosser, Susan

AU - Stark, Marshall

AU - Smith, Margaret C M

N1 - © 2014 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).

PY - 2014/7/29

Y1 - 2014/7/29

N2 - Within the last twenty-five years bacteriophage integrases have rapidly risen to prominence as genetic tools for a wide range of applications from basic cloning to genome engineering. Serine integrases such as that from ϕC31 and its relatives have found an especially wide-range of applications within diverse micro-organisms right through to multi-cellular eukaryotes. Here we review the mechanisms of the two major families of integrases, the tyrosine and serine integrases, and the advantages and disadvantages of each type as they are applied in genome engineering and synthetic biology. In particular, we focus on the new areas of metabolic pathway construction and optimisation, bio-computing, heterologous expression and multiplexed assembly techniques. Integrases are versatile and efficient tools that can be used in conjunction with the various extant molecular biology tools to streamline the synthetic biology production line.

AB - Within the last twenty-five years bacteriophage integrases have rapidly risen to prominence as genetic tools for a wide range of applications from basic cloning to genome engineering. Serine integrases such as that from ϕC31 and its relatives have found an especially wide-range of applications within diverse micro-organisms right through to multi-cellular eukaryotes. Here we review the mechanisms of the two major families of integrases, the tyrosine and serine integrases, and the advantages and disadvantages of each type as they are applied in genome engineering and synthetic biology. In particular, we focus on the new areas of metabolic pathway construction and optimisation, bio-computing, heterologous expression and multiplexed assembly techniques. Integrases are versatile and efficient tools that can be used in conjunction with the various extant molecular biology tools to streamline the synthetic biology production line.

U2 - 10.1016/j.jmb.2014.05.014

DO - 10.1016/j.jmb.2014.05.014

M3 - Article

C2 - 24857859

SN - 0022-2836

VL - 426

SP - 2703

EP - 2716

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 15

ER -