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From the same journal

New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA

Research output: Contribution to journalArticle

Published copy (DOI)

Author(s)

  • Nathan Wales
  • Christian Carøe
  • Marcela Sandoval-Velasco
  • Cristina Gamba
  • Ross Barnett
  • José Alfredo Samaniego
  • Jazmín Ramos Madrigal
  • Ludovic Orlando
  • M. Thomas P. Gilbert

Department/unit(s)

Publication details

JournalBiotechniques
DatePublished - 1 Dec 2015
Issue number6
Volume59
Number of pages4
Pages (from-to)368-371
Original languageEnglish

Abstract

An innovative single-stranded DNA (ssDNA) library preparation method has sparked great interest among ancient DNA (aDNA) researchers, especially after reports of endogenous DNA content increases >20-fold in some samples. To investigate the behavior of this method, we generated ssDNA and conventional double-stranded DNA (dsDNA) libraries from 23 ancient and historic plant and animal specimens. We found ssDNA library preparation substantially increased endogenous content when dsDNA libraries contained <3% endogenous DNA, but this enrichment is less pronounced when dsDNA preparations successfully recover short endogenous DNA fragments (mean size < 70 bp). Our findings can help researchers determine when to utilize the time-and resource-intensive ssDNA library preparation method.

    Research areas

  • Ancient DNA, DNA library preparation, Next-generation sequencing, Paleogenomics

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