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New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA

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New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA. / Wales, Nathan; Carøe, Christian; Sandoval-Velasco, Marcela; Gamba, Cristina; Barnett, Ross; Samaniego, José Alfredo; Madrigal, Jazmín Ramos; Orlando, Ludovic; Gilbert, M. Thomas P.

In: Biotechniques, Vol. 59, No. 6, 114364, 01.12.2015, p. 368-371.

Research output: Contribution to journalArticle

Harvard

Wales, N, Carøe, C, Sandoval-Velasco, M, Gamba, C, Barnett, R, Samaniego, JA, Madrigal, JR, Orlando, L & Gilbert, MTP 2015, 'New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA', Biotechniques, vol. 59, no. 6, 114364, pp. 368-371. https://doi.org/10.2144/000114364

APA

Wales, N., Carøe, C., Sandoval-Velasco, M., Gamba, C., Barnett, R., Samaniego, J. A., ... Gilbert, M. T. P. (2015). New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA. Biotechniques, 59(6), 368-371. [114364]. https://doi.org/10.2144/000114364

Vancouver

Wales N, Carøe C, Sandoval-Velasco M, Gamba C, Barnett R, Samaniego JA et al. New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA. Biotechniques. 2015 Dec 1;59(6):368-371. 114364. https://doi.org/10.2144/000114364

Author

Wales, Nathan ; Carøe, Christian ; Sandoval-Velasco, Marcela ; Gamba, Cristina ; Barnett, Ross ; Samaniego, José Alfredo ; Madrigal, Jazmín Ramos ; Orlando, Ludovic ; Gilbert, M. Thomas P. / New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA. In: Biotechniques. 2015 ; Vol. 59, No. 6. pp. 368-371.

Bibtex - Download

@article{7eb56d3b90874297b2ccc89673ac8208,
title = "New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA",
abstract = "An innovative single-stranded DNA (ssDNA) library preparation method has sparked great interest among ancient DNA (aDNA) researchers, especially after reports of endogenous DNA content increases >20-fold in some samples. To investigate the behavior of this method, we generated ssDNA and conventional double-stranded DNA (dsDNA) libraries from 23 ancient and historic plant and animal specimens. We found ssDNA library preparation substantially increased endogenous content when dsDNA libraries contained <3{\%} endogenous DNA, but this enrichment is less pronounced when dsDNA preparations successfully recover short endogenous DNA fragments (mean size < 70 bp). Our findings can help researchers determine when to utilize the time-and resource-intensive ssDNA library preparation method.",
keywords = "Ancient DNA, DNA library preparation, Next-generation sequencing, Paleogenomics",
author = "Nathan Wales and Christian Car{\o}e and Marcela Sandoval-Velasco and Cristina Gamba and Ross Barnett and Samaniego, {Jos{\'e} Alfredo} and Madrigal, {Jazm{\'i}n Ramos} and Ludovic Orlando and Gilbert, {M. Thomas P.}",
year = "2015",
month = "12",
day = "1",
doi = "10.2144/000114364",
language = "English",
volume = "59",
pages = "368--371",
journal = "Biotechniques",
issn = "0736-6205",
publisher = "Eaton Publishing Company",
number = "6",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA

AU - Wales, Nathan

AU - Carøe, Christian

AU - Sandoval-Velasco, Marcela

AU - Gamba, Cristina

AU - Barnett, Ross

AU - Samaniego, José Alfredo

AU - Madrigal, Jazmín Ramos

AU - Orlando, Ludovic

AU - Gilbert, M. Thomas P.

PY - 2015/12/1

Y1 - 2015/12/1

N2 - An innovative single-stranded DNA (ssDNA) library preparation method has sparked great interest among ancient DNA (aDNA) researchers, especially after reports of endogenous DNA content increases >20-fold in some samples. To investigate the behavior of this method, we generated ssDNA and conventional double-stranded DNA (dsDNA) libraries from 23 ancient and historic plant and animal specimens. We found ssDNA library preparation substantially increased endogenous content when dsDNA libraries contained <3% endogenous DNA, but this enrichment is less pronounced when dsDNA preparations successfully recover short endogenous DNA fragments (mean size < 70 bp). Our findings can help researchers determine when to utilize the time-and resource-intensive ssDNA library preparation method.

AB - An innovative single-stranded DNA (ssDNA) library preparation method has sparked great interest among ancient DNA (aDNA) researchers, especially after reports of endogenous DNA content increases >20-fold in some samples. To investigate the behavior of this method, we generated ssDNA and conventional double-stranded DNA (dsDNA) libraries from 23 ancient and historic plant and animal specimens. We found ssDNA library preparation substantially increased endogenous content when dsDNA libraries contained <3% endogenous DNA, but this enrichment is less pronounced when dsDNA preparations successfully recover short endogenous DNA fragments (mean size < 70 bp). Our findings can help researchers determine when to utilize the time-and resource-intensive ssDNA library preparation method.

KW - Ancient DNA

KW - DNA library preparation

KW - Next-generation sequencing

KW - Paleogenomics

UR - http://www.scopus.com/inward/record.url?scp=84949802001&partnerID=8YFLogxK

U2 - 10.2144/000114364

DO - 10.2144/000114364

M3 - Article

VL - 59

SP - 368

EP - 371

JO - Biotechniques

T2 - Biotechniques

JF - Biotechniques

SN - 0736-6205

IS - 6

M1 - 114364

ER -