Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche

Marielle Balzano; Maria De Grandis; Thien-Phong Vu Manh; Lionel Chasson; Florence Bardin; Anne Farina; Arnauld Serge; Ghislain Bidaut; Pierre Charbord; Leonard Herault; Anne-Laure Bailly; Amandine Cartier-Michaud; Annie Boned; Marc Dalod; Estelle Duprez; Paul Gilbert Genever; Mark Christopher Coles; Marc Bajenoff; Luc Xerri; Michel Aurrand-Lions; Claudine Schiff; Stephane J.C. Mancini

doi:10.1016/j.celrep.2019.02.065

Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche

Marielle Balzano, Maria De Grandis, Thien-Phong Vu Manh, Lionel Chasson, Florence Bardin, Anne Farina, Arnauld Serge, Ghislain Bidaut, Pierre Charbord, Leonard Herault, Anne-Laure Bailly, Amandine Cartier-Michaud, Annie Boned, Marc Dalod, Estelle Duprez, Paul Gilbert Genever, Mark Christopher Coles, Marc Bajenoff, Luc Xerri, Michel Aurrand-LionsClaudine Schiff, Stephane J.C. Mancini

Research output: Contribution to journal › Article › peer-review

Abstract

In the bone marrow, CXCL12 and IL-7 are essential for B cell differentiation, whereas hematopoietic stem cell (HSC) maintenance requires SCF and CXCL12. Peri-sinusoidal stromal (PSS) cells are the main source of IL-7, but their characterization as a pro-B cell niche remains limited. Here, we characterize pro-B cell supporting stromal cells and decipher the interaction network allowing pro-B cell retention. Preferential contacts are found between pro-B cells and PSS cells, which homogeneously express HSC and B cell niche genes. Furthermore, pro-B cells are frequently located in the vicinity of HSCs in the same niche. Using an interactome bioinformatics pipeline, we identify Nidogen-1 as essential for pro-B cell retention in the peri-sinusoidal niche as confirmed in Nidogen-1−/− mice. Finally, human pro-B cells and hematopoietic progenitors are observed close to similar IL-7+ stromal cells. Thus, a multispecific niche exists in mouse and human supporting both early progenitors and committed hematopoietic lineages.

Original language	English
Pages (from-to)	3257–3271
Number of pages	24
Journal	Cell reports
Volume	26
DOIs	https://doi.org/10.1016/j.celrep.2019.02.065
Publication status	Published - 19 Mar 2019

Bibliographical note

Access to Document

10.1016/j.celrep.2019.02.065

Nidogen-1 Contributes to the Interaction NetworkInvolved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche
© 2019 The Author(s).
Final published version, 7.13 MBLicence: CC BY-NC-ND

Mark Christopher Coles
- mc542york.acuk
- Centre for Immunology and Infection - Professor, Former employee
Person: Academic

Cite this

Balzano, M., De Grandis, M., Vu Manh, T.-P., Chasson, L., Bardin, F., Farina, A., Serge, A., Bidaut, G., Charbord, P., Herault, L., Bailly, A.-L., Cartier-Michaud, A., Boned, A., Dalod, M., Duprez, E., Genever, P. G., Coles, M. C., Bajenoff, M., Xerri, L., ... Mancini, S. J. C. (2019). Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche. Cell reports, 26, 3257–3271. https://doi.org/10.1016/j.celrep.2019.02.065

@article{6936b76c3c0845c0a661ea5b0470f926,

title = "Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche",

abstract = "In the bone marrow, CXCL12 and IL-7 are essential for B cell differentiation, whereas hematopoietic stem cell (HSC) maintenance requires SCF and CXCL12. Peri-sinusoidal stromal (PSS) cells are the main source of IL-7, but their characterization as a pro-B cell niche remains limited. Here, we characterize pro-B cell supporting stromal cells and decipher the interaction network allowing pro-B cell retention. Preferential contacts are found between pro-B cells and PSS cells, which homogeneously express HSC and B cell niche genes. Furthermore, pro-B cells are frequently located in the vicinity of HSCs in the same niche. Using an interactome bioinformatics pipeline, we identify Nidogen-1 as essential for pro-B cell retention in the peri-sinusoidal niche as confirmed in Nidogen-1−/− mice. Finally, human pro-B cells and hematopoietic progenitors are observed close to similar IL-7+ stromal cells. Thus, a multispecific niche exists in mouse and human supporting both early progenitors and committed hematopoietic lineages.",

author = "Marielle Balzano and {De Grandis}, Maria and {Vu Manh}, Thien-Phong and Lionel Chasson and Florence Bardin and Anne Farina and Arnauld Serge and Ghislain Bidaut and Pierre Charbord and Leonard Herault and Anne-Laure Bailly and Amandine Cartier-Michaud and Annie Boned and Marc Dalod and Estelle Duprez and Genever, {Paul Gilbert} and Coles, {Mark Christopher} and Marc Bajenoff and Luc Xerri and Michel Aurrand-Lions and Claudine Schiff and Mancini, {Stephane J.C.}",

note = "{\textcopyright} 2019 The Author(s).",

year = "2019",

month = mar,

day = "19",

doi = "10.1016/j.celrep.2019.02.065",

language = "English",

volume = "26",

pages = "3257–3271",

journal = "Cell reports",

issn = "2211-1247",

publisher = "Cell Press",

}

Balzano, M, De Grandis, M, Vu Manh, T-P, Chasson, L, Bardin, F, Farina, A, Serge, A, Bidaut, G, Charbord, P, Herault, L, Bailly, A-L, Cartier-Michaud, A, Boned, A, Dalod, M, Duprez, E, Genever, PG , Coles, MC, Bajenoff, M, Xerri, L, Aurrand-Lions, M, Schiff, C & Mancini, SJC 2019, 'Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche', Cell reports, vol. 26, pp. 3257–3271. https://doi.org/10.1016/j.celrep.2019.02.065

TY - JOUR

T1 - Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche

AU - Balzano, Marielle

AU - De Grandis, Maria

AU - Vu Manh, Thien-Phong

AU - Chasson, Lionel

AU - Bardin, Florence

AU - Farina, Anne

AU - Serge, Arnauld

AU - Bidaut, Ghislain

AU - Charbord, Pierre

AU - Herault, Leonard

AU - Bailly, Anne-Laure

AU - Cartier-Michaud, Amandine

AU - Boned, Annie

AU - Dalod, Marc

AU - Duprez, Estelle

AU - Genever, Paul Gilbert

AU - Coles, Mark Christopher

AU - Bajenoff, Marc

AU - Xerri, Luc

AU - Aurrand-Lions, Michel

AU - Schiff, Claudine

AU - Mancini, Stephane J.C.

PY - 2019/3/19

Y1 - 2019/3/19

N2 - In the bone marrow, CXCL12 and IL-7 are essential for B cell differentiation, whereas hematopoietic stem cell (HSC) maintenance requires SCF and CXCL12. Peri-sinusoidal stromal (PSS) cells are the main source of IL-7, but their characterization as a pro-B cell niche remains limited. Here, we characterize pro-B cell supporting stromal cells and decipher the interaction network allowing pro-B cell retention. Preferential contacts are found between pro-B cells and PSS cells, which homogeneously express HSC and B cell niche genes. Furthermore, pro-B cells are frequently located in the vicinity of HSCs in the same niche. Using an interactome bioinformatics pipeline, we identify Nidogen-1 as essential for pro-B cell retention in the peri-sinusoidal niche as confirmed in Nidogen-1−/− mice. Finally, human pro-B cells and hematopoietic progenitors are observed close to similar IL-7+ stromal cells. Thus, a multispecific niche exists in mouse and human supporting both early progenitors and committed hematopoietic lineages.

AB - In the bone marrow, CXCL12 and IL-7 are essential for B cell differentiation, whereas hematopoietic stem cell (HSC) maintenance requires SCF and CXCL12. Peri-sinusoidal stromal (PSS) cells are the main source of IL-7, but their characterization as a pro-B cell niche remains limited. Here, we characterize pro-B cell supporting stromal cells and decipher the interaction network allowing pro-B cell retention. Preferential contacts are found between pro-B cells and PSS cells, which homogeneously express HSC and B cell niche genes. Furthermore, pro-B cells are frequently located in the vicinity of HSCs in the same niche. Using an interactome bioinformatics pipeline, we identify Nidogen-1 as essential for pro-B cell retention in the peri-sinusoidal niche as confirmed in Nidogen-1−/− mice. Finally, human pro-B cells and hematopoietic progenitors are observed close to similar IL-7+ stromal cells. Thus, a multispecific niche exists in mouse and human supporting both early progenitors and committed hematopoietic lineages.

U2 - 10.1016/j.celrep.2019.02.065

DO - 10.1016/j.celrep.2019.02.065

M3 - Article

SN - 2211-1247

VL - 26

SP - 3257

EP - 3271

JO - Cell reports

JF - Cell reports

ER -

Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche

Abstract

Bibliographical note

Access to Document

Profiles

Mark Christopher Coles

Cite this