Nodulation and nitrogen fixation by Mimosa spp. in the Cerrado and Caatinga biomes of Brazil

TY - JOUR

T1 - Nodulation and nitrogen fixation by Mimosa spp. in the Cerrado and Caatinga biomes of Brazil

AU - Bueno dos Reis Jr, Fábio

AU - Simon, Marcelo F.

AU - Gross, Eduardo

AU - Boddey, Robert M.

AU - Elliott, Geoffrey N.

AU - Neto, Nicolau E.

AU - Loureiro, M. de Fatima

AU - Queiroz, Luciano P. de

AU - Scotti, Maria Rita

AU - Chen, Wen-Ming

AU - Norén, Agneta

AU - Rubio, Maria C.

AU - Faria, Sergio M. de

AU - Bontemps, Cyril

AU - Goi, Silvia R.

AU - Young, J. Peter W.

AU - Sprent, Janet I.

AU - James, Euan K.

N1 - 10.1111/j.1469-8137.2010.03267.x

PY - 2010/6

Y1 - 2010/6

N2 - An extensive survey of nodulation in the legume genus Mimosa was undertaken in two major biomes in Brazil, the Cerrado and the Caatinga, in both of which there are high degrees of endemicity of the genus. Nodules were collected from 67 of the 70 Mimosa spp. found. Thirteen of the species were newly reported as nodulating. Nodules were examined by light and electron microscopy, and all except for M. gatesiae had a structure typical of effective Mimosa nodules. The endosymbiotic bacteria in nodules from all of the Mimosa spp. were identified as Burkholderia via immunolabelling with an antibody against Burkholderia phymatum STM815. Twenty of the 23 Mimosa nodules tested were shown to contain nitrogenase by immunolabelling with an antibody to the nitrogenase Fe- (nifH) protein, and using the 03B415N (15N natural abundance) technique, contributions by biological N2 fixation of up to 60% of total plant N were calculated for Caatinga Mimosa spp. It is concluded that nodulation in Mimosa is a generic character, and that the preferred symbionts of Brazilian species are Burkholderia. This is the first study to demonstrate N2 fixation by beta-rhizobial symbioses in the field.

AB - An extensive survey of nodulation in the legume genus Mimosa was undertaken in two major biomes in Brazil, the Cerrado and the Caatinga, in both of which there are high degrees of endemicity of the genus. Nodules were collected from 67 of the 70 Mimosa spp. found. Thirteen of the species were newly reported as nodulating. Nodules were examined by light and electron microscopy, and all except for M. gatesiae had a structure typical of effective Mimosa nodules. The endosymbiotic bacteria in nodules from all of the Mimosa spp. were identified as Burkholderia via immunolabelling with an antibody against Burkholderia phymatum STM815. Twenty of the 23 Mimosa nodules tested were shown to contain nitrogenase by immunolabelling with an antibody to the nitrogenase Fe- (nifH) protein, and using the 03B415N (15N natural abundance) technique, contributions by biological N2 fixation of up to 60% of total plant N were calculated for Caatinga Mimosa spp. It is concluded that nodulation in Mimosa is a generic character, and that the preferred symbionts of Brazilian species are Burkholderia. This is the first study to demonstrate N2 fixation by beta-rhizobial symbioses in the field.

KW - beta-rhizobia

KW - Burkholderia

KW - campo rupestre

KW - Cupriavidus taiwanensis

KW - nitrogenase

KW - 15N natural abundance

KW - Pantanal

KW - NATURAL-ABUNDANCE TECHNIQUE

KW - RALSTONIA-TAIWANENSIS

KW - SP-NOV.

KW - NODULE BACTERIA

KW - ROOT-NODULES

KW - BURKHOLDERIA

KW - LEGUMES

KW - PLANT

KW - N-15

KW - SYMBIONTS

UR - http://www.scopus.com/inward/record.url?scp=77953934426&partnerID=8YFLogxK

U2 - 10.1111/j.1469-8137.2010.03267.x

DO - 10.1111/j.1469-8137.2010.03267.x

M3 - Article

SN - 1469-8137

VL - 186

SP - 934

EP - 946

JO - New Phytologist

JF - New Phytologist

IS - 4

ER -