Nodulation of Sesbania species by Rhizobium (Agrobacterium) strain IRBG74 and other rhizobia

Stephen P. Cummings; Prasad Gyaneshwar; Pablo Vinuesa; Frank T. Farruggia; Mitchell Andrews; David Humphry; Geoffrey N. Elliott; Andrew Nelson; Caroline Orr; Deborah Pettitt; Gopit R. Shah; Scott R. Santos; Hari B. Krishnan; David Odee; Fatima M. S. Moreira; Janet I. Sprent; J. Peter W. Young; Euan K. James

doi:10.1111/j.1462-2920.2009.01975.x

Nodulation of Sesbania species by Rhizobium (Agrobacterium) strain IRBG74 and other rhizobia

Stephen P. Cummings, Prasad Gyaneshwar, Pablo Vinuesa, Frank T. Farruggia, Mitchell Andrews, David Humphry, Geoffrey N. Elliott, Andrew Nelson, Caroline Orr, Deborah Pettitt, Gopit R. Shah, Scott R. Santos, Hari B. Krishnan, David Odee, Fatima M. S. Moreira, Janet I. Sprent, J. Peter W. Young, Euan K. James

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

P>Concatenated sequence analysis with 16S rRNA, rpoB and fusA genes identified a bacterial strain (IRBG74) isolated from root nodules of the aquatic legume Sesbania cannabina as a close relative of the plant pathogen Rhizobium radiobacter (syn. Agrobacterium tumefaciens). However, DNA:DNA hybridization with R. radiobacter, R. rubi, R. vitis and R. huautlense gave only 44%, 5%, 8% and 8% similarity respectively, suggesting that IRBG74 is potentially a new species. Additionally, it contained no vir genes and lacked tumour-forming ability, but harboured a sym-plasmid containing nifH and nodA genes similar to those in other Sesbania symbionts. Indeed, IRBG74 effectively nodulated S. cannabina and seven other Sesbania spp. that nodulate with Ensifer (Sinorhizobium)/Rhizobium strains with similar nodA genes to IRBG74, but not species that nodulate with Azorhizobium or Mesorhizobium. Light and electron microscopy revealed that IRBG74 infected Sesbania spp. via lateral root junctions under flooded conditions, but via root hairs under non-flooded conditions. Thus, IRBG74 is the first confirmed legume-nodulating symbiont from the Rhizobium (Agrobacterium) clade. Cross-inoculation studies with various Sesbania symbionts showed that S. cannabina could form fully effective symbioses with strains in the genera Rhizobium and Ensifer, only ineffective ones with Azorhizobium strains, and either partially effective (Mesorhizobium huakii) or ineffective (Mesorhizobium plurifarium) symbioses with Mesorhizobium. These data are discussed in terms of the molecular phylogeny of Sesbania and its symbionts.

Original language	English
Pages (from-to)	2510-2525
Number of pages	16
Journal	Environmental Microbiology
Volume	11
Issue number	10
DOIs	https://doi.org/10.1111/j.1462-2920.2009.01975.x
Publication status	Published - Oct 2009

Bibliographical note

10.1111/j.1462-2920.2009.01975.x

Keywords

TOLERANT LEGUME NODULATION
AZORHIZOBIUM-CAULINODANS
SINORHIZOBIUM-SAHELI
MAXIMUM-LIKELIHOOD
RENATURATION RATES
DNA HYBRIDIZATION
GENETIC DIVERSITY
STEM NODULES
NIFH GENES
SP-NOV

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Cite this

Cummings, S. P., Gyaneshwar, P., Vinuesa, P., Farruggia, F. T., Andrews, M., Humphry, D., Elliott, G. N., Nelson, A., Orr, C., Pettitt, D., Shah, G. R., Santos, S. R., Krishnan, H. B., Odee, D., Moreira, F. M. S., Sprent, J. I., Young, J. P. W., & James, E. K. (2009). Nodulation of Sesbania species by Rhizobium (Agrobacterium) strain IRBG74 and other rhizobia. Environmental Microbiology, 11(10), 2510-2525. https://doi.org/10.1111/j.1462-2920.2009.01975.x

@article{474ef49ac8ca47ebac8e9be283f68ffe,

title = "Nodulation of Sesbania species by Rhizobium (Agrobacterium) strain IRBG74 and other rhizobia",

abstract = "P>Concatenated sequence analysis with 16S rRNA, rpoB and fusA genes identified a bacterial strain (IRBG74) isolated from root nodules of the aquatic legume Sesbania cannabina as a close relative of the plant pathogen Rhizobium radiobacter (syn. Agrobacterium tumefaciens). However, DNA:DNA hybridization with R. radiobacter, R. rubi, R. vitis and R. huautlense gave only 44%, 5%, 8% and 8% similarity respectively, suggesting that IRBG74 is potentially a new species. Additionally, it contained no vir genes and lacked tumour-forming ability, but harboured a sym-plasmid containing nifH and nodA genes similar to those in other Sesbania symbionts. Indeed, IRBG74 effectively nodulated S. cannabina and seven other Sesbania spp. that nodulate with Ensifer (Sinorhizobium)/Rhizobium strains with similar nodA genes to IRBG74, but not species that nodulate with Azorhizobium or Mesorhizobium. Light and electron microscopy revealed that IRBG74 infected Sesbania spp. via lateral root junctions under flooded conditions, but via root hairs under non-flooded conditions. Thus, IRBG74 is the first confirmed legume-nodulating symbiont from the Rhizobium (Agrobacterium) clade. Cross-inoculation studies with various Sesbania symbionts showed that S. cannabina could form fully effective symbioses with strains in the genera Rhizobium and Ensifer, only ineffective ones with Azorhizobium strains, and either partially effective (Mesorhizobium huakii) or ineffective (Mesorhizobium plurifarium) symbioses with Mesorhizobium. These data are discussed in terms of the molecular phylogeny of Sesbania and its symbionts.",

keywords = "TOLERANT LEGUME NODULATION, AZORHIZOBIUM-CAULINODANS, SINORHIZOBIUM-SAHELI, MAXIMUM-LIKELIHOOD, RENATURATION RATES, DNA HYBRIDIZATION, GENETIC DIVERSITY, STEM NODULES, NIFH GENES, SP-NOV",

author = "Cummings, {Stephen P.} and Prasad Gyaneshwar and Pablo Vinuesa and Farruggia, {Frank T.} and Mitchell Andrews and David Humphry and Elliott, {Geoffrey N.} and Andrew Nelson and Caroline Orr and Deborah Pettitt and Shah, {Gopit R.} and Santos, {Scott R.} and Krishnan, {Hari B.} and David Odee and Moreira, {Fatima M. S.} and Sprent, {Janet I.} and Young, {J. Peter W.} and James, {Euan K.}",

note = "10.1111/j.1462-2920.2009.01975.x",

year = "2009",

month = oct,

doi = "10.1111/j.1462-2920.2009.01975.x",

language = "English",

volume = "11",

pages = "2510--2525",

journal = "Environmental Microbiology",

publisher = "Wiley-Blackwell",

number = "10",

}

Cummings, SP, Gyaneshwar, P, Vinuesa, P, Farruggia, FT, Andrews, M, Humphry, D, Elliott, GN, Nelson, A, Orr, C, Pettitt, D, Shah, GR, Santos, SR, Krishnan, HB, Odee, D, Moreira, FMS, Sprent, JI, Young, JPW & James, EK 2009, 'Nodulation of Sesbania species by Rhizobium (Agrobacterium) strain IRBG74 and other rhizobia', Environmental Microbiology, vol. 11, no. 10, pp. 2510-2525. https://doi.org/10.1111/j.1462-2920.2009.01975.x

TY - JOUR

T1 - Nodulation of Sesbania species by Rhizobium (Agrobacterium) strain IRBG74 and other rhizobia

AU - Cummings, Stephen P.

AU - Gyaneshwar, Prasad

AU - Vinuesa, Pablo

AU - Farruggia, Frank T.

AU - Andrews, Mitchell

AU - Humphry, David

AU - Elliott, Geoffrey N.

AU - Nelson, Andrew

AU - Orr, Caroline

AU - Pettitt, Deborah

AU - Shah, Gopit R.

AU - Santos, Scott R.

AU - Krishnan, Hari B.

AU - Odee, David

AU - Moreira, Fatima M. S.

AU - Sprent, Janet I.

AU - Young, J. Peter W.

AU - James, Euan K.

N1 - 10.1111/j.1462-2920.2009.01975.x

PY - 2009/10

Y1 - 2009/10

N2 - P>Concatenated sequence analysis with 16S rRNA, rpoB and fusA genes identified a bacterial strain (IRBG74) isolated from root nodules of the aquatic legume Sesbania cannabina as a close relative of the plant pathogen Rhizobium radiobacter (syn. Agrobacterium tumefaciens). However, DNA:DNA hybridization with R. radiobacter, R. rubi, R. vitis and R. huautlense gave only 44%, 5%, 8% and 8% similarity respectively, suggesting that IRBG74 is potentially a new species. Additionally, it contained no vir genes and lacked tumour-forming ability, but harboured a sym-plasmid containing nifH and nodA genes similar to those in other Sesbania symbionts. Indeed, IRBG74 effectively nodulated S. cannabina and seven other Sesbania spp. that nodulate with Ensifer (Sinorhizobium)/Rhizobium strains with similar nodA genes to IRBG74, but not species that nodulate with Azorhizobium or Mesorhizobium. Light and electron microscopy revealed that IRBG74 infected Sesbania spp. via lateral root junctions under flooded conditions, but via root hairs under non-flooded conditions. Thus, IRBG74 is the first confirmed legume-nodulating symbiont from the Rhizobium (Agrobacterium) clade. Cross-inoculation studies with various Sesbania symbionts showed that S. cannabina could form fully effective symbioses with strains in the genera Rhizobium and Ensifer, only ineffective ones with Azorhizobium strains, and either partially effective (Mesorhizobium huakii) or ineffective (Mesorhizobium plurifarium) symbioses with Mesorhizobium. These data are discussed in terms of the molecular phylogeny of Sesbania and its symbionts.

AB - P>Concatenated sequence analysis with 16S rRNA, rpoB and fusA genes identified a bacterial strain (IRBG74) isolated from root nodules of the aquatic legume Sesbania cannabina as a close relative of the plant pathogen Rhizobium radiobacter (syn. Agrobacterium tumefaciens). However, DNA:DNA hybridization with R. radiobacter, R. rubi, R. vitis and R. huautlense gave only 44%, 5%, 8% and 8% similarity respectively, suggesting that IRBG74 is potentially a new species. Additionally, it contained no vir genes and lacked tumour-forming ability, but harboured a sym-plasmid containing nifH and nodA genes similar to those in other Sesbania symbionts. Indeed, IRBG74 effectively nodulated S. cannabina and seven other Sesbania spp. that nodulate with Ensifer (Sinorhizobium)/Rhizobium strains with similar nodA genes to IRBG74, but not species that nodulate with Azorhizobium or Mesorhizobium. Light and electron microscopy revealed that IRBG74 infected Sesbania spp. via lateral root junctions under flooded conditions, but via root hairs under non-flooded conditions. Thus, IRBG74 is the first confirmed legume-nodulating symbiont from the Rhizobium (Agrobacterium) clade. Cross-inoculation studies with various Sesbania symbionts showed that S. cannabina could form fully effective symbioses with strains in the genera Rhizobium and Ensifer, only ineffective ones with Azorhizobium strains, and either partially effective (Mesorhizobium huakii) or ineffective (Mesorhizobium plurifarium) symbioses with Mesorhizobium. These data are discussed in terms of the molecular phylogeny of Sesbania and its symbionts.

KW - TOLERANT LEGUME NODULATION

KW - AZORHIZOBIUM-CAULINODANS

KW - SINORHIZOBIUM-SAHELI

KW - MAXIMUM-LIKELIHOOD

KW - RENATURATION RATES

KW - DNA HYBRIDIZATION

KW - GENETIC DIVERSITY

KW - STEM NODULES

KW - NIFH GENES

KW - SP-NOV

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U2 - 10.1111/j.1462-2920.2009.01975.x

DO - 10.1111/j.1462-2920.2009.01975.x

M3 - Article

VL - 11

SP - 2510

EP - 2525

JO - Environmental Microbiology

JF - Environmental Microbiology

IS - 10

ER -

Nodulation of Sesbania species by Rhizobium (Agrobacterium) strain IRBG74 and other rhizobia

Abstract

Bibliographical note

Keywords

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