Non-coordinate expression of peroxisome biogenesis, beta-oxidation and glyoxylate cycle genes in mature Arabidopsis plants

W L Charlton; B Johnson; I A Graham; A Baker

doi:10.1007/s00299-004-0879-7

Non-coordinate expression of peroxisome biogenesis, beta-oxidation and glyoxylate cycle genes in mature Arabidopsis plants

W L Charlton, B Johnson, I A Graham, A Baker

CNAP

Research output: Contribution to journal › Article › peer-review

Abstract

The expression of three genes that encode proteins involved in peroxisome biogenesis, beta-oxidation and the glyoxylate cycle was studied in Arabidopsis plants by fusing their promoter regions to the reporter gene luciferase. Malate synthase showed an extremely restricted pattern of expression, being detected only in young seedlings and the root tips of older plants. PEX1 and 3-ketoacyl thiolase (PED1) were expressed in roots, mature leaves, stems and flowers. However, only thiolase was up-regulated by starvation. Immunoblotting confirmed that neither malate synthase nor the other unique glyoxylate cycle enzyme isocitrate lyase are expressed in senescent leaves. These results indicate that, in contrast to cucumber, pumpkin and barley, the glyoxylate cycle does not play a role in the recycling of carbon from the turnover of membrane lipids during senescence and starvation in Arabidopsis.

Original language	English
Pages (from-to)	647-653
Number of pages	7
Journal	Plant cell reports
Volume	23
Issue number	9
DOIs	https://doi.org/10.1007/s00299-004-0879-7
Publication status	Published - Feb 2005

Keywords

glyoxylate cycle
beta-oxidation
peroxisomes
luciferase
starvation
CASTOR BEAN ENDOSPERM
MALATE-SYNTHASE
PHOSPHOENOLPYRUVATE CARBOXYKINASE
RESERVE MOBILIZATION
CUCUMBER COTYLEDONS
PUMPKIN COTYLEDONS
ISOCITRATE LYASE
BARLEY LEAVES
ACID
SENESCENCE

Access to Document

10.1007/s00299-004-0879-7

Cite this

@article{045150f3c6e841378ff6070f7d00c195,

title = "Non-coordinate expression of peroxisome biogenesis, beta-oxidation and glyoxylate cycle genes in mature Arabidopsis plants",

abstract = "The expression of three genes that encode proteins involved in peroxisome biogenesis, beta-oxidation and the glyoxylate cycle was studied in Arabidopsis plants by fusing their promoter regions to the reporter gene luciferase. Malate synthase showed an extremely restricted pattern of expression, being detected only in young seedlings and the root tips of older plants. PEX1 and 3-ketoacyl thiolase (PED1) were expressed in roots, mature leaves, stems and flowers. However, only thiolase was up-regulated by starvation. Immunoblotting confirmed that neither malate synthase nor the other unique glyoxylate cycle enzyme isocitrate lyase are expressed in senescent leaves. These results indicate that, in contrast to cucumber, pumpkin and barley, the glyoxylate cycle does not play a role in the recycling of carbon from the turnover of membrane lipids during senescence and starvation in Arabidopsis.",

keywords = "glyoxylate cycle, beta-oxidation, peroxisomes, luciferase, starvation, CASTOR BEAN ENDOSPERM, MALATE-SYNTHASE, PHOSPHOENOLPYRUVATE CARBOXYKINASE, RESERVE MOBILIZATION, CUCUMBER COTYLEDONS, PUMPKIN COTYLEDONS, ISOCITRATE LYASE, BARLEY LEAVES, ACID, SENESCENCE",

author = "Charlton, {W L} and B Johnson and Graham, {I A} and A Baker",

year = "2005",

month = feb,

doi = "10.1007/s00299-004-0879-7",

language = "English",

volume = "23",

pages = "647--653",

journal = "Plant cell reports",

issn = "0721-7714",

publisher = "Springer",

number = "9",

}

TY - JOUR

T1 - Non-coordinate expression of peroxisome biogenesis, beta-oxidation and glyoxylate cycle genes in mature Arabidopsis plants

AU - Charlton, W L

AU - Johnson, B

AU - Graham, I A

AU - Baker, A

PY - 2005/2

Y1 - 2005/2

N2 - The expression of three genes that encode proteins involved in peroxisome biogenesis, beta-oxidation and the glyoxylate cycle was studied in Arabidopsis plants by fusing their promoter regions to the reporter gene luciferase. Malate synthase showed an extremely restricted pattern of expression, being detected only in young seedlings and the root tips of older plants. PEX1 and 3-ketoacyl thiolase (PED1) were expressed in roots, mature leaves, stems and flowers. However, only thiolase was up-regulated by starvation. Immunoblotting confirmed that neither malate synthase nor the other unique glyoxylate cycle enzyme isocitrate lyase are expressed in senescent leaves. These results indicate that, in contrast to cucumber, pumpkin and barley, the glyoxylate cycle does not play a role in the recycling of carbon from the turnover of membrane lipids during senescence and starvation in Arabidopsis.

AB - The expression of three genes that encode proteins involved in peroxisome biogenesis, beta-oxidation and the glyoxylate cycle was studied in Arabidopsis plants by fusing their promoter regions to the reporter gene luciferase. Malate synthase showed an extremely restricted pattern of expression, being detected only in young seedlings and the root tips of older plants. PEX1 and 3-ketoacyl thiolase (PED1) were expressed in roots, mature leaves, stems and flowers. However, only thiolase was up-regulated by starvation. Immunoblotting confirmed that neither malate synthase nor the other unique glyoxylate cycle enzyme isocitrate lyase are expressed in senescent leaves. These results indicate that, in contrast to cucumber, pumpkin and barley, the glyoxylate cycle does not play a role in the recycling of carbon from the turnover of membrane lipids during senescence and starvation in Arabidopsis.

KW - glyoxylate cycle

KW - beta-oxidation

KW - peroxisomes

KW - luciferase

KW - starvation

KW - CASTOR BEAN ENDOSPERM

KW - MALATE-SYNTHASE

KW - PHOSPHOENOLPYRUVATE CARBOXYKINASE

KW - RESERVE MOBILIZATION

KW - CUCUMBER COTYLEDONS

KW - PUMPKIN COTYLEDONS

KW - ISOCITRATE LYASE

KW - BARLEY LEAVES

KW - ACID

KW - SENESCENCE

U2 - 10.1007/s00299-004-0879-7

DO - 10.1007/s00299-004-0879-7

M3 - Article

SN - 0721-7714

VL - 23

SP - 647

EP - 653

JO - Plant cell reports

JF - Plant cell reports

IS - 9

ER -