Novel activity-based probes for broad-spectrum profiling of retaining β-exoglucosidases in situ and in vivo

Wouter W. Kallemeijn, Kah-Yee Li, Martin D. Witte, André R A Marques, Jan Aten, Saskia Scheij, Jianbing Jiang, Lianne I. Willems, Tineke M. Voorn-Brouwer, Cindy P.A.A. van Roomen, Roelof Ottenhoff, Rolf G. Boot, Hans Van Den Elst, Marthe T C Walvoort, Bogdan I. Florea, Jeroen D C Codée, Gijsbert A. Van Der Marel, Johannes M F G Aerts*, Herman S. Overkleeft

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A high-end label: Cyclophellitol aziridine-type activity-based probes allow for ultra-sensitive visualization of mammalian β-glucosidases (GBA1, GBA2, GBA3, and LPH) as well as several non-mammalian β-glucosidases (see picture). These probes offer new ways to study β-exoglucosidases, and configurational isomers of the cyclophellitol aziridine core may give activity-based probes targeting other retaining glycosidase families.

Original languageEnglish
Pages (from-to)12529-12533
Number of pages5
JournalAngewandte Chemie International Edition
Volume51
Issue number50
DOIs
Publication statusPublished - 7 Dec 2012

Keywords

  • activity-based probes
  • fluorescent probes
  • glucocerebrosidase
  • glycosidases
  • proteomics

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