Novel urothelium specific gene expression identified by differential display reverse transcriptase-polymerase chain reaction

TY - JOUR

T1 - Novel urothelium specific gene expression identified by differential display reverse transcriptase-polymerase chain reaction

AU - Hall, G. D.

AU - Smith, B.

AU - Weeks, R. J.

AU - Selby, P. J.

AU - Southgate, J.

AU - Chester, J. D.

PY - 2006/1/1

Y1 - 2006/1/1

N2 - Purpose: Understanding the molecular basis of differential gene expression among different tissues at various developmental stages and in neoplastic transformation is an important biological goal. The potential clinical applications of this improved understanding are more precise diagnosis of disease, prediction of prognosis, novel targeted therapies and prediction of response to therapy. Materials and Methods: Differential display reverse transcriptase-polymerase chain reaction was used to compare gene expression in bovine urothelium to that in autologous lung, esophagus, liver and spleen. Products that appeared to have urothelial specific expression were sequenced and assessed for homology with known sequences. Ribonuclease protection assays were used to further confirm the expression pattern. Results: A total of 32 discrete cDNAs were identified, including 3 products from genes known to be urothelium specific in their expression, 16 with significant homology to bovine, human or mouse expressed sequence tags and 5 with no sequence homology to any currently available sequence. Urothelium specific mRNA expression was confirmed for 3 genes by ribonuclease protection assays and one (Udd06) was further characterized as a urea transporter. Conclusions: The use of differential display reverse transcriptase-polymerase chain reaction and other complementary techniques for parallel gene expression analysis will permit the complete characterization of the urothelial transcriptome and help identify potential molecular targets for rationally targeted therapy.

AB - Purpose: Understanding the molecular basis of differential gene expression among different tissues at various developmental stages and in neoplastic transformation is an important biological goal. The potential clinical applications of this improved understanding are more precise diagnosis of disease, prediction of prognosis, novel targeted therapies and prediction of response to therapy. Materials and Methods: Differential display reverse transcriptase-polymerase chain reaction was used to compare gene expression in bovine urothelium to that in autologous lung, esophagus, liver and spleen. Products that appeared to have urothelial specific expression were sequenced and assessed for homology with known sequences. Ribonuclease protection assays were used to further confirm the expression pattern. Results: A total of 32 discrete cDNAs were identified, including 3 products from genes known to be urothelium specific in their expression, 16 with significant homology to bovine, human or mouse expressed sequence tags and 5 with no sequence homology to any currently available sequence. Urothelium specific mRNA expression was confirmed for 3 genes by ribonuclease protection assays and one (Udd06) was further characterized as a urea transporter. Conclusions: The use of differential display reverse transcriptase-polymerase chain reaction and other complementary techniques for parallel gene expression analysis will permit the complete characterization of the urothelial transcriptome and help identify potential molecular targets for rationally targeted therapy.

KW - Bladder

KW - Gene therapy

KW - Polymerase chain reaction

KW - Urea transporter

KW - Urothelium

UR - http://www.scopus.com/inward/record.url?scp=29144484015&partnerID=8YFLogxK

U2 - 10.1016/S0022-5347(05)00006-6

DO - 10.1016/S0022-5347(05)00006-6

M3 - Article

C2 - 16406938

AN - SCOPUS:29144484015

SN - 0022-5347

VL - 175

SP - 337

EP - 342

JO - British journal of urology

JF - British journal of urology

IS - 1

ER -