Nucleotide sequence of a portion of the camphor-degrading gene cluster from Rhodococcus sp NCIMB 9784

G A Roberts, G Grogan, N J Turner, S L Flitsch

Research output: Contribution to journalArticlepeer-review

Abstract

Rhodococcus sp. NCIMB 9784 is a camphor-degrading Gram-positive organism originally isolated from activated sewage sludge. A 5.4 kbp portion of a proposed camphor degradation gene cluster from this organism was cloned and its nucleotide sequence determined. Four open reading frames (ORFs) were identified encoding proteins possibly involved in camphor metabolism; sequence alignment of the translation products suggested that the ORFs encode for a ferredoxin reductase, acyl-CoA ligase, epimerase and an acyl-CoA dehydrogenase. The last three activities are thought to be involved in the poorly understood late stage of camphor degradation. Our findings are entirely consistent with the proposed formation of a branched 9-carbon acid intermediate (3,4,4-trimethyl-5-oxo- trans -2-hexenoic acid) which has been isolated from the fermentation broth of camphor-grown cells.

Original languageEnglish
Pages (from-to)96-103
Number of pages8
JournalDNA SEQUENCE
Volume15
Issue number2
DOIs
Publication statusPublished - Apr 2004

Keywords

  • camphor
  • ferredoxin reductase
  • acyl-CoA ligase
  • acyl-CoA dehydrogenase
  • RETRO-CLAISEN REACTION
  • PSEUDOMONAS-PUTIDA
  • CROTONASE SUPERFAMILY
  • BETA-DIKETONES
  • DESYMMETRIZATION
  • CYTOCHROME-P450
  • MONOOXYGENASES
  • IDENTIFICATION
  • DEHYDROGENASE
  • DEGRADATION

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