Abstract
A method has been developed for fast evaluation of transesterification activity of immobilized lipases using microlitre and submicrolitre volumes of substrate solutions. The model reaction (acylation of isopropanol with vinyl acetate) is catalyzed by microbial lipases immobilized on ceramic particles, diatoms or acrylic resin, packed into a Teflon tube connected to fused-silica capillary tubing. The substrate solution is pumped through the microreactor and the product of transesterification, acetaldehyde, quantified on capillary by UV absorbance at 280 nm. Using this system in the continuous-flow mode, comparisons were made of transesterification catalysed by PS-C II lipase in two different solvent mixtures. Acetonitrile was found to be a compatible solvent that can be used as a solubilizer without suppressing enzymatic activity. The method has been used to compare conversions at a fixed flow rate using a single pass of substrate through packed beds containing similar to 0.2 mg of supported enzyme. Spatial distributions of the product have been visualized using a complementary metal oxide semiconductor (CMOS) imaging detector in conjunction with the microreactor system operated in the stopped-flow mode. (c) 2006 Elsevier B.V. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 508-518 |
| Number of pages | 11 |
| Journal | JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY |
| Volume | 126 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1 Dec 2006 |
Keywords
- CMOS
- enzyme assay
- enzymatic microreactor
- imaging
- immobilized lipase
- transesterification
- MULTIPLEXED CAPILLARY-ELECTROPHORESIS
- ORGANIC-SOLVENTS
- ESTERASES
- DETECTOR
- ARRAY