ON THE APPLICATION OF DIRECT-METHODS TO OLIGONUCLEOTIDE CRYSTALLOGRAPHY

S R Hubbard; R J Greenall; M M Woolfson

ON THE APPLICATION OF DIRECT-METHODS TO OLIGONUCLEOTIDE CRYSTALLOGRAPHY

S R Hubbard, R J Greenall, M M Woolfson

Physics

Research output: Contribution to journal › Article › peer-review

Abstract

The direct methods program SAYTAN was applied to simulated data at various resolutions from three oligonucleotides. Success in solving the structures was found to depend more upon the resolution of the data than upon errors in the data or the complexity of the structure. Collecting the data at a reduced temperature has little effect, unless it alters the mosaicity of the crystal or changes the resolution of the data. The presence of a heavy atom dramatically improved the phase refinement, particularly at low resolution.

Original language	English
Pages (from-to)	833-841
Number of pages	9
Journal	Acta crystallographica section d-Biological crystallography
Volume	50
Publication status	Published - 1 Nov 1994

Keywords

PHASE-RELATIONSHIPS
COMPLEX STRUCTURES
MOLECULAR-STRUCTURE
CRYSTAL-STRUCTURE
B-DNA
SMALL PROTEIN
SALT
CONFORMATION
D(CPGPCPG)
DAUNOMYCIN

Cite this

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title = "ON THE APPLICATION OF DIRECT-METHODS TO OLIGONUCLEOTIDE CRYSTALLOGRAPHY",

abstract = "The direct methods program SAYTAN was applied to simulated data at various resolutions from three oligonucleotides. Success in solving the structures was found to depend more upon the resolution of the data than upon errors in the data or the complexity of the structure. Collecting the data at a reduced temperature has little effect, unless it alters the mosaicity of the crystal or changes the resolution of the data. The presence of a heavy atom dramatically improved the phase refinement, particularly at low resolution.",

keywords = "PHASE-RELATIONSHIPS, COMPLEX STRUCTURES, MOLECULAR-STRUCTURE, CRYSTAL-STRUCTURE, B-DNA, SMALL PROTEIN, SALT, CONFORMATION, D(CPGPCPG), DAUNOMYCIN",

author = "Hubbard, {S R} and Greenall, {R J} and Woolfson, {M M}",

year = "1994",

month = nov,

day = "1",

language = "English",

volume = "50",

pages = "833--841",

journal = "Acta crystallographica section d-Biological crystallography",

issn = "0907-4449",

publisher = "International Union of Crystallography",

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TY - JOUR

T1 - ON THE APPLICATION OF DIRECT-METHODS TO OLIGONUCLEOTIDE CRYSTALLOGRAPHY

AU - Hubbard, S R

AU - Greenall, R J

AU - Woolfson, M M

PY - 1994/11/1

Y1 - 1994/11/1

N2 - The direct methods program SAYTAN was applied to simulated data at various resolutions from three oligonucleotides. Success in solving the structures was found to depend more upon the resolution of the data than upon errors in the data or the complexity of the structure. Collecting the data at a reduced temperature has little effect, unless it alters the mosaicity of the crystal or changes the resolution of the data. The presence of a heavy atom dramatically improved the phase refinement, particularly at low resolution.

AB - The direct methods program SAYTAN was applied to simulated data at various resolutions from three oligonucleotides. Success in solving the structures was found to depend more upon the resolution of the data than upon errors in the data or the complexity of the structure. Collecting the data at a reduced temperature has little effect, unless it alters the mosaicity of the crystal or changes the resolution of the data. The presence of a heavy atom dramatically improved the phase refinement, particularly at low resolution.

KW - PHASE-RELATIONSHIPS

KW - COMPLEX STRUCTURES

KW - MOLECULAR-STRUCTURE

KW - CRYSTAL-STRUCTURE

KW - B-DNA

KW - SMALL PROTEIN

KW - SALT

KW - CONFORMATION

KW - D(CPGPCPG)

KW - DAUNOMYCIN

M3 - Article

SN - 0907-4449

VL - 50

SP - 833

EP - 841

JO - Acta crystallographica section d-Biological crystallography

JF - Acta crystallographica section d-Biological crystallography

ER -