On the roles of AA15 lytic polysaccharide monooxygenases derived from the termite Coptotermes gestroi

TY - JOUR

T1 - On the roles of AA15 lytic polysaccharide monooxygenases derived from the termite Coptotermes gestroi

AU - Walton, Paul Howard

AU - Lourenco Franco Cairo, Joao Paulo

AU - Cannella, David

AU - Oliveira, Leandro

AU - Goncalves, Thiago

AU - Rubio, Marcelo

AU - Terrasan, Cesar R.F.

AU - Tramontina, Robson

AU - Mofatto, Luciana S.

AU - Carazzolle, Marcelo F.

AU - Garcia, Wanius

AU - Felby, Claus

AU - Damasio, André

AU - Squina, Fabio M.

N1 - Funding Information: We would like to acknowledge professors Luisa Ciano and Gideon Davies as well as Dr. Alessandro Paradisi for supporting on experiments and for comments on the manuscript. We also would like to acknowledge Dr. Magdalena Calusinska for kindly provide the sequences of LPMOs AA15 from termites Cortaritermes sp. and Macrotermes natalensis. This research was supported by grants from Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo - FAPESP (FMS 2015/50590?4; JPLFC 2016/09950?0; 2017/11952?3; WG 2017/17275?3; TAG 2017/16089?1; AD 2015/50612?8 and 2017/22669?0) and Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq. (FMS 428527/2018?3 and 305740/2017?2; WG 422132/2018?7; LCO 442352/2014?0; AD 404654/2018?5 and 304816/2017?5). We would like to acknowledge the MASS facility from Brazilian Biosciences National Laboratory (LNBio ? CNPEM) Campinas, SP, Brazil; the Analytic Center from the Institute of Chemistry from the State University of Campinas, Campinas, SP, Brazil; and the Technology Facility, Department of Biology, University of York, York, United Kingdom. The molecular dynamics simulations were performed at the Center for Scientific Computing (NCC/GridUNESP) of the S?o Paulo State University (UNESP) and CENAPAD-SP (Centro Nacional de Processamento de Alto Desempenho em S?o Paulo), project UNICAMP/FINEP-MCTII. Funding Information: We would like to acknowledge professors Luisa Ciano and Gideon Davies as well as Dr. Alessandro Paradisi for supporting on experiments and for comments on the manuscript. We also would like to acknowledge Dr. Magdalena Calusinska for kindly provide the sequences of LPMOs AA15 from termites Cortaritermes sp. and Macrotermes natalensis. This research was supported by grants from Fundação de Amparo à Pesquisa do Estado de São Paulo - FAPESP ( FMS 2015/50590–4 ; JPLFC 2016/09950–0 ; 2017/11952–3 ; WG 2017/17275–3 ; TAG 2017/16089–1 ; AD 2015/50612–8 and 2017/22669–0 ) and Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq. ( FMS 428527/2018–3 and 305740/2017–2 ; WG 422132/2018–7 ; LCO 442352/2014–0 ; AD 404654/2018–5 and 304816/2017–5 ). We would like to acknowledge the MASS facility from Brazilian Biosciences National Laboratory (LNBio – CNPEM) Campinas, SP, Brazil; the Analytic Center from the Institute of Chemistry from the State University of Campinas, Campinas, SP, Brazil; and the Technology Facility, Department of Biology, University of York, York, United Kingdom. The molecular dynamics simulations were performed at the Center for Scientific Computing (NCC/GridUNESP) of the São Paulo State University (UNESP) and CENAPAD-SP (Centro Nacional de Processamento de Alto Desempenho em São Paulo), project UNICAMP/FINEP-MCTII. Publisher Copyright: © 2020 Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/1/6

Y1 - 2021/1/6

N2 - Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes which catalyze the oxidative cleavage of polysaccharides. LPMOs belonging to family 15 in the Auxiliary Activity (AA) class from the Carbohydrate-Active Enzyme database are found widespread across the Tree of Life, including viruses, algae, oomycetes and animals. Recently, two AA15s from the firebrat Thermobia domestica were reported to have oxidative activity, one towards cellulose or chitin and the other towards chitin, signalling that AA15 LPMOs from insects potentially have different biochemical functions. Herein, we report the identification and characterization of two family AA15 members from the lower termite Coptotermes gestroi. Addition of Cu(II) to CgAA15a or CgAA15b had a thermostabilizing effect on both.

AB - Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes which catalyze the oxidative cleavage of polysaccharides. LPMOs belonging to family 15 in the Auxiliary Activity (AA) class from the Carbohydrate-Active Enzyme database are found widespread across the Tree of Life, including viruses, algae, oomycetes and animals. Recently, two AA15s from the firebrat Thermobia domestica were reported to have oxidative activity, one towards cellulose or chitin and the other towards chitin, signalling that AA15 LPMOs from insects potentially have different biochemical functions. Herein, we report the identification and characterization of two family AA15 members from the lower termite Coptotermes gestroi. Addition of Cu(II) to CgAA15a or CgAA15b had a thermostabilizing effect on both.

KW - AA15

KW - CAZymes

KW - Chitin

KW - Chitinases

KW - LPMOs

KW - Termites

UR - http://www.scopus.com/inward/record.url?scp=85098891992&partnerID=8YFLogxK

U2 - 10.1016/j.jinorgbio.2020.111316

DO - 10.1016/j.jinorgbio.2020.111316

M3 - Article

AN - SCOPUS:85098891992

SN - 0162-0134

VL - 216

JO - JOURNAL OF INORGANIC BIOCHEMISTRY

JF - JOURNAL OF INORGANIC BIOCHEMISTRY

M1 - 111316

ER -