By the same authors

From the same journal

On-line low-volume transesterification-based assay for immobilized lipases

Research output: Contribution to journalArticle

Published copy (DOI)

Author(s)

Department/unit(s)

Publication details

JournalJOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
DatePublished - 1 Dec 2006
Issue number4
Volume126
Number of pages11
Pages (from-to)508-518
Original languageEnglish

Abstract

A method has been developed for fast evaluation of transesterification activity of immobilized lipases using microlitre and submicrolitre volumes of substrate solutions. The model reaction (acylation of isopropanol with vinyl acetate) is catalyzed by microbial lipases immobilized on ceramic particles, diatoms or acrylic resin, packed into a Teflon tube connected to fused-silica capillary tubing. The substrate solution is pumped through the microreactor and the product of transesterification, acetaldehyde, quantified on capillary by UV absorbance at 280 nm. Using this system in the continuous-flow mode, comparisons were made of transesterification catalysed by PS-C II lipase in two different solvent mixtures. Acetonitrile was found to be a compatible solvent that can be used as a solubilizer without suppressing enzymatic activity. The method has been used to compare conversions at a fixed flow rate using a single pass of substrate through packed beds containing similar to 0.2 mg of supported enzyme. Spatial distributions of the product have been visualized using a complementary metal oxide semiconductor (CMOS) imaging detector in conjunction with the microreactor system operated in the stopped-flow mode. (c) 2006 Elsevier B.V. All rights reserved.

    Research areas

  • CMOS, enzyme assay, enzymatic microreactor, imaging, immobilized lipase, transesterification, MULTIPLEXED CAPILLARY-ELECTROPHORESIS, ORGANIC-SOLVENTS, ESTERASES, DETECTOR, ARRAY

Discover related content

Find related publications, people, projects, datasets and more using interactive charts.

View graph of relations