Palladium-unleashed proteins: gentle aldehyde decaging for site-selective protein modification

Robin Louis Brabham, Richard James Spears, Julia Walton, Swati Tyagi, Edward Lemke, Martin Anthony Fascione

Research output: Contribution to journalArticlepeer-review


Protein bioconjugation frequently makes use of aldehydes as reactive handles, with methods for their installation being highly valued. Here a new, powerful strategy to unmask a reactive protein aldehyde is presented. A genetically encoded caged glyoxyl aldehyde, situated in solvent-accessible locations, can be rapidly decade through treatment with just one equivalent of allylpalladium(II) chloride dimer at physiological pH. The protein aldehyde can undergo subsequent oxime ligation for site-selective protein modification. Quick yet mild conditions, orthogonality and powerful exposed reactivity make this strategy of great potential in protein modification.
Original languageEnglish
Pages (from-to)1501-1504
Number of pages4
JournalChemical Communications
Publication statusPublished - 6 Feb 2018

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