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From the same journal

pBaSysBioll: an integrative plasmid generating gfp transcriptional fusions for high-throughput analysis of gene expression in Bacillus subtilis

Research output: Contribution to journalArticle

Published copy (DOI)

Author(s)

  • Eric Botella
  • Mark Fogg
  • Matthieu Jules
  • Sjouke Piersma
  • Geoff Doherty
  • Annette Hansen
  • Emma. L. Denham
  • Ludovic Le Chat
  • Patrick Veiga
  • Kirra Bailey
  • Peter J. Lewis
  • Jan Maarten van Dijl
  • Stephane Aymerich
  • Anthony J. Wilkinson
  • Kevin M. Devine

Department/unit(s)

Publication details

JournalMicrobiology
DatePublished - Jun 2010
Issue number6
Volume156
Number of pages9
Pages (from-to)1600-1608
Original languageEnglish

Abstract

Plasmid pBaSysBioll was constructed for high-throughput analysis of gene expression in Bacillus subtilis. It is an integrative plasmid with a ligation-independent cloning (LIC) site, allowing the generation of transcriptional gfpmut3 fusions with desired promoters. Integration is by a Campbell-type event and is non-mutagenic, placing the fusion at the homologous chromosomal locus. Using phoA, murAA, gapB, ptsG and cggR promoters that are responsive to phosphate availability, growth rate and carbon source, we show that detailed profiles of promoter activity can be established, with responses to changing conditions being measurable within 1 min of the stimulus. This makes pBaSysBioll a highly versatile tool for real-time gene expression analysis in growing cells of B. subtilis.

    Research areas

  • LIGATION-INDEPENDENT CLONING, GREEN FLUORESCENT PROTEIN, PHOPR 2-COMPONENT SYSTEMS, KINETICS, BACTERIA, GLUCOSE, TARGET, YYCFG

Projects

  • BaSysBio

    Project: Research project (funded)Research

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