Pectate lyase 10A from Pseudomonas cellulosa is a modular enzyme containing a family 2a carbohydrate-binding module

I E Brown, M H Mallen, S J Charnock, G J Davies, G W Black

Research output: Contribution to journalArticlepeer-review

Abstract

Pectate lyase 10A (Pel10A) enzyme from Pseudomonas cellulosa is composed of 649 residues and has a molecular mass of 68.5 kDa. Sequence analysis revealed that Pel10A contained a signal peptide and two serine-rich linker sequences that separate three modules. Sequence similarity was seen between the 9.2 kDa N-terminal module of Pel10A and Family 2a carbohydrate-binding modules (CBMs). This N-terminal module of Pel10A was shown to encode an independently functional module with affinity to crystalline cellulose. A high sequence identity of 66% was seen between the 14.2 kDa central module of Pel 10A and the functionally uncharacterized central modules of the xylan-degrading enzymes endoxylanase 10B, arabinofuranosidase 62C and esterase 1D, also from P, cellulosa. The 35.8 kDa C-terminal module of Pel10A was shown to have 30 and 36% identities with the family 10 pectate lyases from Azospirillum irakense and an alkaliphilic strain of Bacillus sp. strain KSM-P15, respectively. This His-tagged C-terminal module of the Pel10A was shown encode an independent catalytic module (Pel10Acm). Pel10Acm was shown to cleave pectate and pectin in an endo-fashion and to have optimal activity at pH 10 and in the presence of 2 mM Ca2+. Highest enzyme activity was detected at 62 degreesC. Pel10Acm was shown to be most active against pectate (i.e. polygalacturonic acid) with progressively less activity against 31. 67 and 89 degrees (degrees), esterified citrus pectins. These data suggest that Pel10A has a preference for sequences of non-esterified galacturonic acid residues. Significantly, Pel10A and the P. cellulosa rhamnogalacturonan Lyase 11A, in the accompanying article [McKie, Vincken, Voragen, van den Broek, Stimson and Gilbert (2001) Biochem. J. 355, 167-177], are the first CBM-containing pectinases described to date.

Original languageEnglish
Pages (from-to)155-165
Number of pages11
JournalBiochemical journal
Volume355
Publication statusPublished - 1 Apr 2001

Keywords

  • beta-elimination
  • esterified pectin
  • homogalacturonan
  • pectinase
  • rhamnogalacturonan
  • FLUORESCENS SUBSP CELLULOSA
  • NUCLEOTIDE-SEQUENCE
  • ERWINIA-CAROTOVORA
  • ANAEROBIC FUNGI
  • DOMAIN
  • GENE
  • ENDOPOLYGALACTURONASE
  • HEMICELLULASES
  • HYDROLYSIS
  • CELLULASES

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