Petrous bone diagenesis: a multi-analytical approach

TY - JOUR

T1 - Petrous bone diagenesis

T2 - a multi-analytical approach

AU - Kontopoulos, Ioannis

AU - Penkman, Kirsty

AU - McAllister, Graeme D.

AU - Lynnerup, Niels

AU - Damgaard, Peter B.

AU - Hansen, Henrik B.

AU - Allentoft, Morten E.

AU - Collins, Matthew J.

N1 - © 2019 Elsevier B.V. All rights reserved. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details.

PY - 2019/3/15

Y1 - 2019/3/15

N2 - The discovery of petrous bone as an excellent repository for ancient biomolecules has been a turning point in biomolecular archaeology, especially in aDNA research, but excessive and uncontrolled sampling could result in loss of this valuable resource for future research. This study reports on the histological (optical microscopy), physical (FTIR-ATR), elemental (CHN) and biochemical (collagen and DNA analysis) preservation of 15 human petrous bones spanning from the c. 2100 BCE to 1850 CE. Through the combined application of a number of diagenetic parameters (general histological index; infrared splitting factor; carbonate/phosphate ratio; amide/phosphate ratio; col wt%; % C, % N and C/N of whole bone and collagen; % endogenous DNA), we provide new insights into petrous bone micromorphological characteristics and diagenesis, and new evidence to enhance screening practices for aDNA and collagen analysis.

AB - The discovery of petrous bone as an excellent repository for ancient biomolecules has been a turning point in biomolecular archaeology, especially in aDNA research, but excessive and uncontrolled sampling could result in loss of this valuable resource for future research. This study reports on the histological (optical microscopy), physical (FTIR-ATR), elemental (CHN) and biochemical (collagen and DNA analysis) preservation of 15 human petrous bones spanning from the c. 2100 BCE to 1850 CE. Through the combined application of a number of diagenetic parameters (general histological index; infrared splitting factor; carbonate/phosphate ratio; amide/phosphate ratio; col wt%; % C, % N and C/N of whole bone and collagen; % endogenous DNA), we provide new insights into petrous bone micromorphological characteristics and diagenesis, and new evidence to enhance screening practices for aDNA and collagen analysis.

KW - aDNA

KW - Bioapatite

KW - Collagen

KW - Diagenesis

KW - Microstructure

KW - Petrous bone

UR - http://www.scopus.com/inward/record.url?scp=85060122834&partnerID=8YFLogxK

U2 - 10.1016/j.palaeo.2019.01.005

DO - 10.1016/j.palaeo.2019.01.005

M3 - Article

AN - SCOPUS:85060122834

SN - 0031-0182

VL - 518

SP - 143

EP - 154

JO - Palaeogeography palaeoclimatology palaeoecology

JF - Palaeogeography palaeoclimatology palaeoecology

ER -