Physiological and genetic dissection of sucrose inputs to the Arabidopsis thaliana circadian system

Koumis Philippou; James Andrew Ronald; Alfredo Sanchez-Villarreal; Amanda M Davis; Seth Jon Davis

doi:10.3390/genes10050334

Physiological and genetic dissection of sucrose inputs to the Arabidopsis thaliana circadian system

Koumis Philippou, James Andrew Ronald, Alfredo Sanchez-Villarreal, Amanda M Davis, Seth Jon Davis

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Abstract

Circadian rhythms allow an organism to synchronize internal physiological responses to the external environment. Perception of external signals such as light and temperature are critical in the entrainment of the oscillator. However, sugar can also act as an entraining signal. In this work we have confirmed that sucrose accelerates circadian period, but this observed effect is dependent on the reporter gene used. This observed response was dependent on sucrose being available during free-running conditions. If sucrose was applied during entrainment, circadian period was only temporally accelerated, if any effect was observed at all. We also found that sucrose acts to stabilize the robustness of circadian period under red light or blue light, in addition to its previously described role in stabilizing the robustness of rhythms in the dark. Finally, we have also found that CCA1 is required for both a short and long-term response of the circadian oscillator to sucrose, while LHY acts to attenuate the effects of sucrose on circadian period. Together, this work highlights new pathways for how sucrose could be signaling to the oscillator and reveals further functional separation of CCA1 and LHY

Original language	English
Number of pages	11
Journal	Genes
Volume	10
Issue number	5
DOIs	https://doi.org/10.3390/genes10050334
Publication status	Published - 2 May 2019

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10.3390/genes10050334

genes-10-00334
© 2019 by the authors.
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title = "Physiological and genetic dissection of sucrose inputs to the Arabidopsis thaliana circadian system",

abstract = "Circadian rhythms allow an organism to synchronize internal physiological responses to the external environment. Perception of external signals such as light and temperature are critical in the entrainment of the oscillator. However, sugar can also act as an entraining signal. In this work we have confirmed that sucrose accelerates circadian period, but this observed effect is dependent on the reporter gene used. This observed response was dependent on sucrose being available during free-running conditions. If sucrose was applied during entrainment, circadian period was only temporally accelerated, if any effect was observed at all. We also found that sucrose acts to stabilize the robustness of circadian period under red light or blue light, in addition to its previously described role in stabilizing the robustness of rhythms in the dark. Finally, we have also found that CCA1 is required for both a short and long-term response of the circadian oscillator to sucrose, while LHY acts to attenuate the effects of sucrose on circadian period. Together, this work highlights new pathways for how sucrose could be signaling to the oscillator and reveals further functional separation of CCA1 and LHY",

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T1 - Physiological and genetic dissection of sucrose inputs to the Arabidopsis thaliana circadian system

AU - Philippou, Koumis

AU - Ronald, James Andrew

AU - Sanchez-Villarreal, Alfredo

AU - Davis, Amanda M

AU - Davis, Seth Jon

PY - 2019/5/2

Y1 - 2019/5/2

N2 - Circadian rhythms allow an organism to synchronize internal physiological responses to the external environment. Perception of external signals such as light and temperature are critical in the entrainment of the oscillator. However, sugar can also act as an entraining signal. In this work we have confirmed that sucrose accelerates circadian period, but this observed effect is dependent on the reporter gene used. This observed response was dependent on sucrose being available during free-running conditions. If sucrose was applied during entrainment, circadian period was only temporally accelerated, if any effect was observed at all. We also found that sucrose acts to stabilize the robustness of circadian period under red light or blue light, in addition to its previously described role in stabilizing the robustness of rhythms in the dark. Finally, we have also found that CCA1 is required for both a short and long-term response of the circadian oscillator to sucrose, while LHY acts to attenuate the effects of sucrose on circadian period. Together, this work highlights new pathways for how sucrose could be signaling to the oscillator and reveals further functional separation of CCA1 and LHY

AB - Circadian rhythms allow an organism to synchronize internal physiological responses to the external environment. Perception of external signals such as light and temperature are critical in the entrainment of the oscillator. However, sugar can also act as an entraining signal. In this work we have confirmed that sucrose accelerates circadian period, but this observed effect is dependent on the reporter gene used. This observed response was dependent on sucrose being available during free-running conditions. If sucrose was applied during entrainment, circadian period was only temporally accelerated, if any effect was observed at all. We also found that sucrose acts to stabilize the robustness of circadian period under red light or blue light, in addition to its previously described role in stabilizing the robustness of rhythms in the dark. Finally, we have also found that CCA1 is required for both a short and long-term response of the circadian oscillator to sucrose, while LHY acts to attenuate the effects of sucrose on circadian period. Together, this work highlights new pathways for how sucrose could be signaling to the oscillator and reveals further functional separation of CCA1 and LHY

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