Plant communities affect arbuscular mycorrhizal fungal diversity and community composition in grassland microcosms

D. Johnson; P.J. Vandenkoornhuyse; J.R. Leake; L. Gilbert; R.E. Booth; J.P. Grime; J.P.W. Young; D.J. Read

doi:10.1046/j.1469-8137.2003.00938.x

Plant communities affect arbuscular mycorrhizal fungal diversity and community composition in grassland microcosms

D. Johnson, P.J. Vandenkoornhuyse, J.R. Leake, L. Gilbert, R.E. Booth, J.P. Grime, J.P.W. Young, D.J. Read

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

The diversity of arbuscular mycorrhizal (AM) fungi was investigated in an unfertilized limestone grassland soil supporting different synthesized vascular plant assemblages that had developed for 3 yr.

The experimental treatments comprised: bare soil; monocultures of the nonmycotrophic sedge Carex flacca; monocultures of the mycotrophic grass Festuca ovina; and a species-rich mixture of four forbs, four grasses and four sedges. The diversity of AM fungi was analysed in roots of Plantago lanceolata bioassay seedlings using terminal-restriction fragment length polymorphism (T-RFLP). The extent of AM colonization, shoot biomass and nitrogen and phosphorus concentrations were also measured.

The AM diversity was affected significantly by the floristic composition of the microcosms and shoot phosphorus concentration was positively correlated with AM diversity. The diversity of AM fungi in P. lanceolata decreased in the order: bare soil > C. flacca > 12 species > F. ovina.

The unexpectedly high diversity in the bare soil and sedge monoculture likely reflects differences in the modes of colonization and sources of inoculum in these treatments compared with the assemblages containing established AM-compatible plants.

Original language	English
Pages (from-to)	503-515
Number of pages	13
Journal	New Phytologist
Volume	161
Issue number	2
DOIs	https://doi.org/10.1046/j.1469-8137.2003.00938.x
Publication status	Published - Feb 2004

Bibliographical note

Keywords

terminal restriction fragment length polymorphism (T-RFLP), arbuscular mycorrhiza (AM), plant functional type
diversity
shoot phosphorus, shoot nitrogen
HYACINTHOIDES NON-SCRIPTA
MOLECULAR DIVERSITY
GLOMUS-MOSSEAE
RIBOSOMAL-RNA
ROOTS
PATTERNS
SOIL
PRODUCTIVITY
COLONIZATION
ECOSYSTEM

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Cite this

@article{f21e70e4aef14f7d89342731c4ed698c,

title = "Plant communities affect arbuscular mycorrhizal fungal diversity and community composition in grassland microcosms",

abstract = "The diversity of arbuscular mycorrhizal (AM) fungi was investigated in an unfertilized limestone grassland soil supporting different synthesized vascular plant assemblages that had developed for 3 yr.The experimental treatments comprised: bare soil; monocultures of the nonmycotrophic sedge Carex flacca; monocultures of the mycotrophic grass Festuca ovina; and a species-rich mixture of four forbs, four grasses and four sedges. The diversity of AM fungi was analysed in roots of Plantago lanceolata bioassay seedlings using terminal-restriction fragment length polymorphism (T-RFLP). The extent of AM colonization, shoot biomass and nitrogen and phosphorus concentrations were also measured.The AM diversity was affected significantly by the floristic composition of the microcosms and shoot phosphorus concentration was positively correlated with AM diversity. The diversity of AM fungi in P. lanceolata decreased in the order: bare soil > C. flacca > 12 species > F. ovina.The unexpectedly high diversity in the bare soil and sedge monoculture likely reflects differences in the modes of colonization and sources of inoculum in these treatments compared with the assemblages containing established AM-compatible plants.",

keywords = "terminal restriction fragment length polymorphism (T-RFLP), arbuscular mycorrhiza (AM), plant functional type, diversity, shoot phosphorus, shoot nitrogen, HYACINTHOIDES NON-SCRIPTA, MOLECULAR DIVERSITY, GLOMUS-MOSSEAE, RIBOSOMAL-RNA, ROOTS, PATTERNS, SOIL, PRODUCTIVITY, COLONIZATION, ECOSYSTEM",

author = "D. Johnson and P.J. Vandenkoornhuyse and J.R. Leake and L. Gilbert and R.E. Booth and J.P. Grime and J.P.W. Young and D.J. Read",

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AU - Johnson, D.

AU - Vandenkoornhuyse, P.J.

AU - Leake, J.R.

AU - Gilbert, L.

AU - Booth, R.E.

AU - Grime, J.P.

AU - Young, J.P.W.

AU - Read, D.J.

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N2 - The diversity of arbuscular mycorrhizal (AM) fungi was investigated in an unfertilized limestone grassland soil supporting different synthesized vascular plant assemblages that had developed for 3 yr.The experimental treatments comprised: bare soil; monocultures of the nonmycotrophic sedge Carex flacca; monocultures of the mycotrophic grass Festuca ovina; and a species-rich mixture of four forbs, four grasses and four sedges. The diversity of AM fungi was analysed in roots of Plantago lanceolata bioassay seedlings using terminal-restriction fragment length polymorphism (T-RFLP). The extent of AM colonization, shoot biomass and nitrogen and phosphorus concentrations were also measured.The AM diversity was affected significantly by the floristic composition of the microcosms and shoot phosphorus concentration was positively correlated with AM diversity. The diversity of AM fungi in P. lanceolata decreased in the order: bare soil > C. flacca > 12 species > F. ovina.The unexpectedly high diversity in the bare soil and sedge monoculture likely reflects differences in the modes of colonization and sources of inoculum in these treatments compared with the assemblages containing established AM-compatible plants.

AB - The diversity of arbuscular mycorrhizal (AM) fungi was investigated in an unfertilized limestone grassland soil supporting different synthesized vascular plant assemblages that had developed for 3 yr.The experimental treatments comprised: bare soil; monocultures of the nonmycotrophic sedge Carex flacca; monocultures of the mycotrophic grass Festuca ovina; and a species-rich mixture of four forbs, four grasses and four sedges. The diversity of AM fungi was analysed in roots of Plantago lanceolata bioassay seedlings using terminal-restriction fragment length polymorphism (T-RFLP). The extent of AM colonization, shoot biomass and nitrogen and phosphorus concentrations were also measured.The AM diversity was affected significantly by the floristic composition of the microcosms and shoot phosphorus concentration was positively correlated with AM diversity. The diversity of AM fungi in P. lanceolata decreased in the order: bare soil > C. flacca > 12 species > F. ovina.The unexpectedly high diversity in the bare soil and sedge monoculture likely reflects differences in the modes of colonization and sources of inoculum in these treatments compared with the assemblages containing established AM-compatible plants.

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KW - COLONIZATION

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