Plasmodium-specific atypical memory B cells are short-lived activated B cells

Damián Pérez-Mazliah; Peter J Gardner; Edina Schweighoffer; Sarah McLaughlin; Caroline Hosking; Irene Tumwine; Randall S Davis; Alexandre J Potocnik; Victor Lj Tybulewicz; Jean Langhorne

doi:10.7554/eLife.39800

Plasmodium-specific atypical memory B cells are short-lived activated B cells

Damián Pérez-Mazliah, Peter J Gardner, Edina Schweighoffer, Sarah McLaughlin, Caroline Hosking, Irene Tumwine, Randall S Davis, Alexandre J Potocnik, Victor Lj Tybulewicz, Jean Langhorne

The Hull York Medical School

Research output: Contribution to journal › Article › peer-review

Abstract

A subset of atypical memory B cells accumulates in malaria and several infections, autoimmune disorders and aging in both humans and mice. It has been suggested these cells are exhausted long-lived memory B cells, and their accumulation may contribute to poor acquisition of long-lasting immunity to certain chronic infections, such as malaria and HIV. Here, we generated an immunoglobulin heavy chain knock-in mouse with a BCR that recognizes MSP1 of the rodent malaria parasite, Plasmodium chabaudi. In combination with a mosquito-initiated P. chabaudi infection, we show that Plasmodium-specific atypical memory B cells are short-lived and disappear upon natural resolution of chronic infection. These cells show features of activation, proliferation, DNA replication, and plasmablasts. Our data demonstrate that Plasmodium-specific atypical memory B cells are not a subset of long-lived memory B cells, but rather short-lived activated cells, and part of a physiologic ongoing B-cell response.

Original language	English
Article number	e39800
Number of pages	28
Journal	eLife
Volume	7
DOIs	https://doi.org/10.7554/eLife.39800
Publication status	Published - 2 Nov 2018

Bibliographical note

Keywords

Animals
B-Lymphocyte Subsets/chemistry
B-Lymphocytes/chemistry
Flow Cytometry
Gene Knock-In Techniques
Immunoglobulin G/genetics
Immunologic Memory
Malaria/immunology
Merozoite Surface Protein 1/immunology
Mice, Inbred BALB C
Mice, Inbred C57BL
Plasmodium chabaudi/immunology
Rodent Diseases/immunology

Access to Document

10.7554/eLife.39800

elife-39800-v2
© 2018, Pérez-Mazliah et al.
Final published version, 3.76 MBLicence: CC BY

Cite this

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title = "Plasmodium-specific atypical memory B cells are short-lived activated B cells",

abstract = "A subset of atypical memory B cells accumulates in malaria and several infections, autoimmune disorders and aging in both humans and mice. It has been suggested these cells are exhausted long-lived memory B cells, and their accumulation may contribute to poor acquisition of long-lasting immunity to certain chronic infections, such as malaria and HIV. Here, we generated an immunoglobulin heavy chain knock-in mouse with a BCR that recognizes MSP1 of the rodent malaria parasite, Plasmodium chabaudi. In combination with a mosquito-initiated P. chabaudi infection, we show that Plasmodium-specific atypical memory B cells are short-lived and disappear upon natural resolution of chronic infection. These cells show features of activation, proliferation, DNA replication, and plasmablasts. Our data demonstrate that Plasmodium-specific atypical memory B cells are not a subset of long-lived memory B cells, but rather short-lived activated cells, and part of a physiologic ongoing B-cell response.",

keywords = "Animals, B-Lymphocyte Subsets/chemistry, B-Lymphocytes/chemistry, Flow Cytometry, Gene Knock-In Techniques, Immunoglobulin G/genetics, Immunologic Memory, Malaria/immunology, Merozoite Surface Protein 1/immunology, Mice, Inbred BALB C, Mice, Inbred C57BL, Plasmodium chabaudi/immunology, Rodent Diseases/immunology",

author = "Dami{\'a}n P{\'e}rez-Mazliah and Gardner, {Peter J} and Edina Schweighoffer and Sarah McLaughlin and Caroline Hosking and Irene Tumwine and Davis, {Randall S} and Potocnik, {Alexandre J} and Tybulewicz, {Victor Lj} and Jean Langhorne",

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doi = "10.7554/eLife.39800",

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AU - Pérez-Mazliah, Damián

AU - Gardner, Peter J

AU - Schweighoffer, Edina

AU - McLaughlin, Sarah

AU - Hosking, Caroline

AU - Tumwine, Irene

AU - Davis, Randall S

AU - Potocnik, Alexandre J

AU - Tybulewicz, Victor Lj

AU - Langhorne, Jean

PY - 2018/11/2

Y1 - 2018/11/2

N2 - A subset of atypical memory B cells accumulates in malaria and several infections, autoimmune disorders and aging in both humans and mice. It has been suggested these cells are exhausted long-lived memory B cells, and their accumulation may contribute to poor acquisition of long-lasting immunity to certain chronic infections, such as malaria and HIV. Here, we generated an immunoglobulin heavy chain knock-in mouse with a BCR that recognizes MSP1 of the rodent malaria parasite, Plasmodium chabaudi. In combination with a mosquito-initiated P. chabaudi infection, we show that Plasmodium-specific atypical memory B cells are short-lived and disappear upon natural resolution of chronic infection. These cells show features of activation, proliferation, DNA replication, and plasmablasts. Our data demonstrate that Plasmodium-specific atypical memory B cells are not a subset of long-lived memory B cells, but rather short-lived activated cells, and part of a physiologic ongoing B-cell response.

AB - A subset of atypical memory B cells accumulates in malaria and several infections, autoimmune disorders and aging in both humans and mice. It has been suggested these cells are exhausted long-lived memory B cells, and their accumulation may contribute to poor acquisition of long-lasting immunity to certain chronic infections, such as malaria and HIV. Here, we generated an immunoglobulin heavy chain knock-in mouse with a BCR that recognizes MSP1 of the rodent malaria parasite, Plasmodium chabaudi. In combination with a mosquito-initiated P. chabaudi infection, we show that Plasmodium-specific atypical memory B cells are short-lived and disappear upon natural resolution of chronic infection. These cells show features of activation, proliferation, DNA replication, and plasmablasts. Our data demonstrate that Plasmodium-specific atypical memory B cells are not a subset of long-lived memory B cells, but rather short-lived activated cells, and part of a physiologic ongoing B-cell response.

KW - Animals

KW - B-Lymphocyte Subsets/chemistry

KW - B-Lymphocytes/chemistry

KW - Flow Cytometry

KW - Gene Knock-In Techniques

KW - Immunoglobulin G/genetics

KW - Immunologic Memory

KW - Malaria/immunology

KW - Merozoite Surface Protein 1/immunology

KW - Mice, Inbred BALB C

KW - Mice, Inbred C57BL

KW - Plasmodium chabaudi/immunology

KW - Rodent Diseases/immunology

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