Pol III binding in six mammals shows conservation among amino acid isotypes despite divergence among tRNA genes

C. Kutter; G.D. Brown; M.D. Wilson; S. Watt; D.T. Odom; Å. Gonçalves; A. Brazma; R.J. White

doi:10.1038/ng.906

Pol III binding in six mammals shows conservation among amino acid isotypes despite divergence among tRNA genes

C. Kutter, G.D. Brown, M.D. Wilson, S. Watt, D.T. Odom, Å. Gonçalves, A. Brazma, R.J. White

Research output: Contribution to journal › Article › peer-review

Abstract

RNA polymerase III (Pol III) transcription of tRNA genes is essential for generating the tRNA adaptor molecules that link genetic sequence and protein translation. By mapping Pol III occupancy genome-wide in mouse, rat, human, macaque, dog and opossum livers, we found that Pol III binding to individual tRNA genes varies substantially in strength and location. However, when we took into account tRNA redundancies by grouping Pol III occupancy into 46 anticodon isoacceptor families or 21 amino acid-based isotype classes, we discovered strong conservation. Similarly, Pol III occupancy of amino acid isotypes is almost invariant among transcriptionally and evolutionarily diverse tissues in mouse. Thus, synthesis of functional tRNA isotypes has been highly constrained, although the usage of individual tRNA genes has evolved rapidly.

Original language	English
Pages (from-to)	948-957
Number of pages	10
Journal	Nature genetics
Volume	43
Issue number	10
DOIs	https://doi.org/10.1038/ng.906
Publication status	Published - 1 Oct 2011

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abstract = "RNA polymerase III (Pol III) transcription of tRNA genes is essential for generating the tRNA adaptor molecules that link genetic sequence and protein translation. By mapping Pol III occupancy genome-wide in mouse, rat, human, macaque, dog and opossum livers, we found that Pol III binding to individual tRNA genes varies substantially in strength and location. However, when we took into account tRNA redundancies by grouping Pol III occupancy into 46 anticodon isoacceptor families or 21 amino acid-based isotype classes, we discovered strong conservation. Similarly, Pol III occupancy of amino acid isotypes is almost invariant among transcriptionally and evolutionarily diverse tissues in mouse. Thus, synthesis of functional tRNA isotypes has been highly constrained, although the usage of individual tRNA genes has evolved rapidly.",

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AU - Brazma, A.

AU - White, R.J.

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Pol III binding in six mammals shows conservation among amino acid isotypes despite divergence among tRNA genes

Abstract

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