POLYMORPHISM IN A DROSOPHILA INDIRECT FLIGHT MUSCLE-SPECIFIC TROPOMYOSIN ISOZYME DOES NOT AFFECT FLIGHT ABILITY

R M Cripps; J C Sparrow

POLYMORPHISM IN A DROSOPHILA INDIRECT FLIGHT MUSCLE-SPECIFIC TROPOMYOSIN ISOZYME DOES NOT AFFECT FLIGHT ABILITY

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

We describe polymorphism in a Drosophila indirect flight muscle-specific tropomyosin isozyme, named TnH-34. Three variants of this protein differ in their mobilities as determined by 1-D and 2-D SDS-PAGE. Meiotic mapping places the polymorphism close to, if not within, the structural gene encoding this tropomyosin isozyme. The most likely site of the mutations is within a single C-terminal exon. Flight-testing of different genotypes reveals that this variation in TnH-34 does not affect flight ability. These results suggest that some sequence variation may be tolerated in this section of the protein and correlate with the variability of this protein in different insect species.

Original language	English
Pages (from-to)	159-168
Number of pages	10
Journal	Biochemical genetics
Volume	30
Issue number	3-4
Publication status	Published - Apr 1992

Keywords

DROSOPHILA
INDIRECT FLIGHT MUSCLE
TROPOMYOSIN
POLYMORPHISM
MYOFIBRILLAR PROTEINS
MELANOGASTER
ELECTROPHORESIS

Cite this

@article{68c7f5ca851442ed83e8be86afe3cf18,

title = "POLYMORPHISM IN A DROSOPHILA INDIRECT FLIGHT MUSCLE-SPECIFIC TROPOMYOSIN ISOZYME DOES NOT AFFECT FLIGHT ABILITY",

abstract = "We describe polymorphism in a Drosophila indirect flight muscle-specific tropomyosin isozyme, named TnH-34. Three variants of this protein differ in their mobilities as determined by 1-D and 2-D SDS-PAGE. Meiotic mapping places the polymorphism close to, if not within, the structural gene encoding this tropomyosin isozyme. The most likely site of the mutations is within a single C-terminal exon. Flight-testing of different genotypes reveals that this variation in TnH-34 does not affect flight ability. These results suggest that some sequence variation may be tolerated in this section of the protein and correlate with the variability of this protein in different insect species.",

keywords = "DROSOPHILA, INDIRECT FLIGHT MUSCLE, TROPOMYOSIN, POLYMORPHISM, MYOFIBRILLAR PROTEINS, MELANOGASTER, ELECTROPHORESIS",

author = "Cripps, {R M} and Sparrow, {J C}",

year = "1992",

month = apr,

language = "English",

volume = "30",

pages = "159--168",

journal = "Biochemical genetics",

issn = "0006-2928",

publisher = "Springer",

number = "3-4",

}

TY - JOUR

T1 - POLYMORPHISM IN A DROSOPHILA INDIRECT FLIGHT MUSCLE-SPECIFIC TROPOMYOSIN ISOZYME DOES NOT AFFECT FLIGHT ABILITY

AU - Cripps, R M

AU - Sparrow, J C

PY - 1992/4

Y1 - 1992/4

N2 - We describe polymorphism in a Drosophila indirect flight muscle-specific tropomyosin isozyme, named TnH-34. Three variants of this protein differ in their mobilities as determined by 1-D and 2-D SDS-PAGE. Meiotic mapping places the polymorphism close to, if not within, the structural gene encoding this tropomyosin isozyme. The most likely site of the mutations is within a single C-terminal exon. Flight-testing of different genotypes reveals that this variation in TnH-34 does not affect flight ability. These results suggest that some sequence variation may be tolerated in this section of the protein and correlate with the variability of this protein in different insect species.

AB - We describe polymorphism in a Drosophila indirect flight muscle-specific tropomyosin isozyme, named TnH-34. Three variants of this protein differ in their mobilities as determined by 1-D and 2-D SDS-PAGE. Meiotic mapping places the polymorphism close to, if not within, the structural gene encoding this tropomyosin isozyme. The most likely site of the mutations is within a single C-terminal exon. Flight-testing of different genotypes reveals that this variation in TnH-34 does not affect flight ability. These results suggest that some sequence variation may be tolerated in this section of the protein and correlate with the variability of this protein in different insect species.

KW - DROSOPHILA

KW - INDIRECT FLIGHT MUSCLE

KW - TROPOMYOSIN

KW - POLYMORPHISM

KW - MYOFIBRILLAR PROTEINS

KW - MELANOGASTER

KW - ELECTROPHORESIS

M3 - Article

SN - 0006-2928

VL - 30

SP - 159

EP - 168

JO - Biochemical genetics

JF - Biochemical genetics

IS - 3-4

ER -