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Pooled extracellular receptor-ligand interaction screening using CRISPR activation.

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JournalGenome biology
DateAccepted/In press - 7 Nov 2018
DatePublished (current) - 26 Nov 2018
Volume19
Number of pages16
Original languageEnglish

Abstract

Extracellular interactions between cell surface receptors are necessary for signaling and adhesion but identifying them remains technically challenging. We describe a cell-based genome-wide approach employing CRISPR activation to identify receptors for a defined ligand. We show receptors for high-affinity antibodies and low-affinity ligands can be unambiguously identified when used in pools or as individual binding probes. We apply this technique to identify ligands for the adhesion G-protein-coupled receptors and show that the Nogo myelin-associated inhibitory proteins are ligands for ADGRB1. This method will enable extracellular receptor-ligand identification on a genome-wide scale.

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