Predicting the effect of chemical factors on the pH of crystallisation trials

Julie C. Wilson; Marko Ristic; Jobie Kirkwood; David Hargreaves; Janet Newman

doi:10.1016/j.isci.2020.101219

Predicting the effect of chemical factors on the pH of crystallisation trials

Julie C. Wilson, Marko Ristic, Jobie Kirkwood, David Hargreaves, Janet Newman

Mathematics

Research output: Contribution to journal › Article › peer-review

Abstract

In macromolecular crystallisation, success is often dependent on the pH of the experiment. However, little is known about the pH of reagents used and it is generally assumed that the pH of the experiment will closely match that of any buffering chemical in the solution. We use a large data set of experimentally measured solution pH values to show that this assumption can be very wrong and generate a model which can be used to successfully predict the overall solution pH of a crystallisation experiment. Further, we investigate the time dependence of the pH of some polyethylene glycol polymers widely used in protein crystallisation under different storage conditions.

Original language	English
Article number	101219
Number of pages	22
Journal	iScience
Volume	23
Issue number	6
Early online date	30 May 2020
DOIs	https://doi.org/10.1016/j.isci.2020.101219
Publication status	Published - 26 Jun 2020

Bibliographical note

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10.1016/j.isci.2020.101219

1-s2.0-S2589004220304041-main
©2020 The Authors.
Final published version, 9.13 MBLicence: CC BY

Cite this

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