Projects per year
Proteomic analysis of dental calculus is emerging as a powerful tool for disease and dietary characterisation of archaeological populations. To better understand the variability in protein results from dental calculus, we analysed 21 samples from three Roman-period populations to compare: 1) the quantity of extracted protein; 2) the number of mass spectral queries; and 3) the number of peptide spectral matches and protein identifications. We found little correlation between the quantity of calculus analysed and total protein identifications, as well as no systematic trends between site location and protein preservation. We identified a wide range of individual variability, which may be associated with the mechanisms of calculus formation and/or post-depositional contamination, in addition to taphonomic factors. Our results suggest dental calculus is indeed a stable, long-term reservoir of proteins as previously reported, but further systematic studies are needed to identify mechanisms associated with protein entrapment and survival in dental calculus.
|Number of pages||14|
|Journal||STAR: Science Technology of Archaeological Research|
|Publication status||Published - 11 Aug 2017|
Bibliographical note© 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
- dental calculus
- destructive analysis
- Shotgun proteomics
- protein preservation
- 3 Finished
Digital Tools for Molecular Conservation and Heritage - CDA with Natural History Museum
Speller, C. F., Collins, M. J., Ramsøe, A., Green, E. & Barnes, I.
1/10/16 → 31/12/20
Project: Other project › Research collaboration
A plaque on both your houses:Exploring the history of urbanisation and infectious diseases through the study of archaeological dental tartar
15/06/15 → 14/06/17
Project: Research project (funded) › Research
C2D2 research 2a - By the Skin of your Teeth: Investigating Chronic Periodontal Disease through History
Speller, C. F., Brockhurst, M., Moir, J., Hofreiter, M. S., Thomas, G. H., Collins, M. J., Young, P. & Fiddyment, S.
1/02/13 → 31/01/14
Project: Other project › Other internal award