Primary prostate stromal cells modulate the morphology and migration of primary prostate epithelial cells in type 1 collagen gels

TY - JOUR

T1 - Primary prostate stromal cells modulate the morphology and migration of primary prostate epithelial cells in type 1 collagen gels

AU - Hall, J A

AU - Maitland, N J

AU - Stower, M

AU - Lang, S H

PY - 2002/1/1

Y1 - 2002/1/1

N2 - The effects of human primary prostatic stromal cells on the migration and morphogenesis of human prostatic epithelial cells, derived from tumor or benign prostatic hyperplasia tissue, were studied using a three-dimensional coculture system. Epithelial cells from tumor or benign tissue migrated efficiently into collagen gels populated with stromal cells from benign tissue. Only epithelial cells from benign prostate formed acinus-like structures that exhibited differentiated prostatic function and strong expression of membrane-associated E-cadherin. In gels populated by stromal cells from tumor tissue, migration of primary prostatic epithelial cells did not occur. In the absence of stromal cells, primary epithelial cells were unable to proliferate. This three-dimensional culture system allows closely controlled manipulation and analysis in vitro of interactions between prostatic epithelial and stromal cells.

AB - The effects of human primary prostatic stromal cells on the migration and morphogenesis of human prostatic epithelial cells, derived from tumor or benign prostatic hyperplasia tissue, were studied using a three-dimensional coculture system. Epithelial cells from tumor or benign tissue migrated efficiently into collagen gels populated with stromal cells from benign tissue. Only epithelial cells from benign prostate formed acinus-like structures that exhibited differentiated prostatic function and strong expression of membrane-associated E-cadherin. In gels populated by stromal cells from tumor tissue, migration of primary prostatic epithelial cells did not occur. In the absence of stromal cells, primary epithelial cells were unable to proliferate. This three-dimensional culture system allows closely controlled manipulation and analysis in vitro of interactions between prostatic epithelial and stromal cells.

KW - BONE-MARROW STROMA

KW - IN-VITRO

KW - BREAST

KW - CANCER

KW - FIBROBLASTS

KW - RAT

M3 - Article

SN - 0008-5472

VL - 62

SP - 58

EP - 62

JO - Cancer research

JF - Cancer research

IS - 1

ER -