Priming of plant innate immunity by rhizobacteria and beta-aminobutyric acid: differences and similarities in regulation

TY - JOUR

T1 - Priming of plant innate immunity by rhizobacteria and beta-aminobutyric acid: differences and similarities in regulation

AU - Van der Ent, Sjoerd

AU - Van Hulten, Marieke

AU - Pozo, Maria J.

AU - Czechowski, Tomasz

AU - Udvardi, Michael K.

AU - Pieterse, Corne M. J.

AU - Ton, Jurriaan

PY - 2009/7

Y1 - 2009/7

N2 - P>Pseudomonas fluorescens WCS417r bacteria and beta-aminobutyric acid can induce disease resistance in Arabidopsis, which is based on priming of defence.In this study, we examined the differences and similarities of WCS417r- and beta-aminobutyric acid-induced priming.Both WCS417r and beta-aminobutyric acid prime for enhanced deposition of callose-rich papillae after infection by the oomycete Hyaloperonospora arabidopsis. This priming is regulated by convergent pathways, which depend on phosphoinositide- and ABA-dependent signalling components. Conversely, induced resistance by WCS417r and beta-aminobutyric acid against the bacterial pathogen Pseudomonas syringae are controlled by distinct NPR1-dependent signalling pathways. As WCS417r and beta-aminobutyric acid prime jasmonate- and salicylate-inducible genes, respectively, we subsequently investigated the role of transcription factors. A quantitative PCR-based genome-wide screen for putative WCS417r- and beta-aminobutyric acid-responsive transcription factor genes revealed distinct sets of priming-responsive genes. Transcriptional analysis of a selection of these genes showed that they can serve as specific markers for priming. Promoter analysis of WRKY genes identified a putative cis-element that is strongly over-represented in promoters of 21 NPR1-dependent, beta-aminobutyric acid-inducible WRKY genes.Our study shows that priming of defence is regulated by different pathways, depending on the inducing agent and the challenging pathogen. Furthermore, we demon-strated that priming is associated with the enhanced expression of transcription factors.New Phytologist (2009) 183: 419-431doi: 10.1111/j.1469-8137.2009.02851.x.

AB - P>Pseudomonas fluorescens WCS417r bacteria and beta-aminobutyric acid can induce disease resistance in Arabidopsis, which is based on priming of defence.In this study, we examined the differences and similarities of WCS417r- and beta-aminobutyric acid-induced priming.Both WCS417r and beta-aminobutyric acid prime for enhanced deposition of callose-rich papillae after infection by the oomycete Hyaloperonospora arabidopsis. This priming is regulated by convergent pathways, which depend on phosphoinositide- and ABA-dependent signalling components. Conversely, induced resistance by WCS417r and beta-aminobutyric acid against the bacterial pathogen Pseudomonas syringae are controlled by distinct NPR1-dependent signalling pathways. As WCS417r and beta-aminobutyric acid prime jasmonate- and salicylate-inducible genes, respectively, we subsequently investigated the role of transcription factors. A quantitative PCR-based genome-wide screen for putative WCS417r- and beta-aminobutyric acid-responsive transcription factor genes revealed distinct sets of priming-responsive genes. Transcriptional analysis of a selection of these genes showed that they can serve as specific markers for priming. Promoter analysis of WRKY genes identified a putative cis-element that is strongly over-represented in promoters of 21 NPR1-dependent, beta-aminobutyric acid-inducible WRKY genes.Our study shows that priming of defence is regulated by different pathways, depending on the inducing agent and the challenging pathogen. Furthermore, we demon-strated that priming is associated with the enhanced expression of transcription factors.New Phytologist (2009) 183: 419-431doi: 10.1111/j.1469-8137.2009.02851.x.

KW - Arabidopsis thaliana

KW - callose

KW - NPR1

KW - priming

KW - transcription factors

KW - WRKY

KW - SYSTEMIC ACQUIRED-RESISTANCE

KW - ARABIDOPSIS TRANSCRIPTION FACTOR

KW - DISEASE RESISTANCE

KW - BIOCONTROL BACTERIA

KW - PATHOGEN INFECTION

KW - GENE-EXPRESSION

KW - SALICYLIC-ACID

KW - DOWNY MILDEW

KW - DEFENSE

KW - PATHWAY

UR - http://www.scopus.com/inward/record.url?scp=67649472796&partnerID=8YFLogxK

U2 - 10.1111/j.1469-8137.2009.02851.x

DO - 10.1111/j.1469-8137.2009.02851.x

M3 - Article

SN - 0028-646X

VL - 183

SP - 419

EP - 431

JO - New Phytologist

JF - New Phytologist

IS - 2

ER -