By the same authors

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Priming of plant innate immunity by rhizobacteria and beta-aminobutyric acid: differences and similarities in regulation

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Author(s)

  • Sjoerd Van der Ent
  • Marieke Van Hulten
  • Maria J. Pozo
  • Tomasz Czechowski
  • Michael K. Udvardi
  • Corne M. J. Pieterse
  • Jurriaan Ton

Department/unit(s)

Publication details

JournalNew Phytologist
DatePublished - Jul 2009
Issue number2
Volume183
Number of pages13
Pages (from-to)419-431
Original languageEnglish

Abstract

P>Pseudomonas fluorescens WCS417r bacteria and beta-aminobutyric acid can induce disease resistance in Arabidopsis, which is based on priming of defence.

In this study, we examined the differences and similarities of WCS417r- and beta-aminobutyric acid-induced priming.

Both WCS417r and beta-aminobutyric acid prime for enhanced deposition of callose-rich papillae after infection by the oomycete Hyaloperonospora arabidopsis. This priming is regulated by convergent pathways, which depend on phosphoinositide- and ABA-dependent signalling components. Conversely, induced resistance by WCS417r and beta-aminobutyric acid against the bacterial pathogen Pseudomonas syringae are controlled by distinct NPR1-dependent signalling pathways. As WCS417r and beta-aminobutyric acid prime jasmonate- and salicylate-inducible genes, respectively, we subsequently investigated the role of transcription factors. A quantitative PCR-based genome-wide screen for putative WCS417r- and beta-aminobutyric acid-responsive transcription factor genes revealed distinct sets of priming-responsive genes. Transcriptional analysis of a selection of these genes showed that they can serve as specific markers for priming. Promoter analysis of WRKY genes identified a putative cis-element that is strongly over-represented in promoters of 21 NPR1-dependent, beta-aminobutyric acid-inducible WRKY genes.

Our study shows that priming of defence is regulated by different pathways, depending on the inducing agent and the challenging pathogen. Furthermore, we demon-strated that priming is associated with the enhanced expression of transcription factors.

New Phytologist (2009) 183: 419-431doi: 10.1111/j.1469-8137.2009.02851.x.

    Research areas

  • Arabidopsis thaliana, callose, NPR1, priming, transcription factors, WRKY, SYSTEMIC ACQUIRED-RESISTANCE, ARABIDOPSIS TRANSCRIPTION FACTOR, DISEASE RESISTANCE, BIOCONTROL BACTERIA, PATHOGEN INFECTION, GENE-EXPRESSION, SALICYLIC-ACID, DOWNY MILDEW, DEFENSE, PATHWAY

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