Process extension by rat CG-4 line progenitor oligodendrocytes: A positively-charged surface promotes a bipolar but not a differentiated multipolar morphology and is not necessary for cell growth.

M G Rumsby; F Afsari; M Stark; E Hughson

Process extension by rat CG-4 line progenitor oligodendrocytes: A positively-charged surface promotes a bipolar but not a differentiated multipolar morphology and is not necessary for cell growth.

M G Rumsby, F Afsari, M Stark, E Hughson

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Rat CG-4 line progenitor oligodendrocytes passaged in neuroblastoma-conditioned medium onto a poly-l-lysine-coated surface adopt a bipolar morphology with long processes and proliferate. On untreated or albumin- or poly-L-glutamate-coated surfaces in the same medium such cells show Little process extension but still proliferate forming clumps of cells in the medium or loosely attached to the substratum. Transfer of these cell clumps onto a poly-L-lysine-coated surface results in cell migration out of the clumps and rapid process elaboration to a bipolar morphology. In differentiation medium containing 1% foetal bovine serum CG-4 line cells extend numerous short processes equally well on poly-L-lysine-, poly-L-glutamate-, albumin-coated or untreated cell culture surfaces and divide more slowly. A positively charged substratum thus may contribute signals initiating development of a bipolar morphology: such signals are not needed for the multiprocess morphology of differentiated CG-4 cells.

Original language	English
Pages (from-to)	101-109
Number of pages	9
Journal	Neuroscience research communications
Volume	23
Issue number	2
Publication status	Published - 1998

Keywords

CG-4 line progenitor oligodendrocytes
substratum charge
bipolar and multipolar morphology
cell growth
differentiation
PROTEIN-KINASE-C
NG2 PROTEOGLYCAN
CULTURED OLIGODENDROCYTES
MYELIN
EXPRESSION
ADHESION
GLYCOPROTEIN
RECEPTOR
PDGF

Cite this

@article{b3cc00cbcbf3492f80659f7189f04c72,

title = "Process extension by rat CG-4 line progenitor oligodendrocytes: A positively-charged surface promotes a bipolar but not a differentiated multipolar morphology and is not necessary for cell growth.",

abstract = "Rat CG-4 line progenitor oligodendrocytes passaged in neuroblastoma-conditioned medium onto a poly-l-lysine-coated surface adopt a bipolar morphology with long processes and proliferate. On untreated or albumin- or poly-L-glutamate-coated surfaces in the same medium such cells show Little process extension but still proliferate forming clumps of cells in the medium or loosely attached to the substratum. Transfer of these cell clumps onto a poly-L-lysine-coated surface results in cell migration out of the clumps and rapid process elaboration to a bipolar morphology. In differentiation medium containing 1% foetal bovine serum CG-4 line cells extend numerous short processes equally well on poly-L-lysine-, poly-L-glutamate-, albumin-coated or untreated cell culture surfaces and divide more slowly. A positively charged substratum thus may contribute signals initiating development of a bipolar morphology: such signals are not needed for the multiprocess morphology of differentiated CG-4 cells.",

keywords = "CG-4 line progenitor oligodendrocytes, substratum charge, bipolar and multipolar morphology, cell growth, differentiation, PROTEIN-KINASE-C, NG2 PROTEOGLYCAN, CULTURED OLIGODENDROCYTES, MYELIN, EXPRESSION, ADHESION, GLYCOPROTEIN, RECEPTOR, PDGF",

author = "Rumsby, {M G} and F Afsari and M Stark and E Hughson",

year = "1998",

language = "English",

volume = "23",

pages = "101--109",

journal = "Neuroscience research communications",

issn = "0893-6609",

publisher = "Wiley-Blackwell",

number = "2",

}

Process extension by rat CG-4 line progenitor oligodendrocytes: A positively-charged surface promotes a bipolar but not a differentiated multipolar morphology and is not necessary for cell growth. / Rumsby, M G; Afsari, F; Stark, M et al.
In: Neuroscience research communications, Vol. 23, No. 2, 1998, p. 101-109.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Process extension by rat CG-4 line progenitor oligodendrocytes: A positively-charged surface promotes a bipolar but not a differentiated multipolar morphology and is not necessary for cell growth.

AU - Rumsby, M G

AU - Afsari, F

AU - Stark, M

AU - Hughson, E

PY - 1998

Y1 - 1998

N2 - Rat CG-4 line progenitor oligodendrocytes passaged in neuroblastoma-conditioned medium onto a poly-l-lysine-coated surface adopt a bipolar morphology with long processes and proliferate. On untreated or albumin- or poly-L-glutamate-coated surfaces in the same medium such cells show Little process extension but still proliferate forming clumps of cells in the medium or loosely attached to the substratum. Transfer of these cell clumps onto a poly-L-lysine-coated surface results in cell migration out of the clumps and rapid process elaboration to a bipolar morphology. In differentiation medium containing 1% foetal bovine serum CG-4 line cells extend numerous short processes equally well on poly-L-lysine-, poly-L-glutamate-, albumin-coated or untreated cell culture surfaces and divide more slowly. A positively charged substratum thus may contribute signals initiating development of a bipolar morphology: such signals are not needed for the multiprocess morphology of differentiated CG-4 cells.

AB - Rat CG-4 line progenitor oligodendrocytes passaged in neuroblastoma-conditioned medium onto a poly-l-lysine-coated surface adopt a bipolar morphology with long processes and proliferate. On untreated or albumin- or poly-L-glutamate-coated surfaces in the same medium such cells show Little process extension but still proliferate forming clumps of cells in the medium or loosely attached to the substratum. Transfer of these cell clumps onto a poly-L-lysine-coated surface results in cell migration out of the clumps and rapid process elaboration to a bipolar morphology. In differentiation medium containing 1% foetal bovine serum CG-4 line cells extend numerous short processes equally well on poly-L-lysine-, poly-L-glutamate-, albumin-coated or untreated cell culture surfaces and divide more slowly. A positively charged substratum thus may contribute signals initiating development of a bipolar morphology: such signals are not needed for the multiprocess morphology of differentiated CG-4 cells.

KW - CG-4 line progenitor oligodendrocytes

KW - substratum charge

KW - bipolar and multipolar morphology

KW - cell growth

KW - differentiation

KW - PROTEIN-KINASE-C

KW - NG2 PROTEOGLYCAN

KW - CULTURED OLIGODENDROCYTES

KW - MYELIN

KW - EXPRESSION

KW - ADHESION

KW - GLYCOPROTEIN

KW - RECEPTOR

KW - PDGF

M3 - Article

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VL - 23

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JO - Neuroscience research communications

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ER -