Production of antifungal iturins from vegetable straw: A combined chemical-bacterial process

TY - JOUR

T1 - Production of antifungal iturins from vegetable straw

T2 - A combined chemical-bacterial process

AU - Yuan, Yuan

AU - Chu, Depeng

AU - Fan, Jiajun

AU - Cui, Zhenzhen

AU - Wang, Rui

AU - Zhang, Han

AU - You, Xiangwei

AU - Li, Yiqiang

AU - Wang, Xiaoqiang

N1 - Funding Information: This work was supported by the Key Laboratory of Tobacco Pest Monitoring & Integrated Management(KLTMMIMT2022-09), by the International Foundation of Tobacco Research Institute of CAAS (IFI202103), by the Major Tobacco Science and Technology Projects(110202101054(LS-14), 20220007, YXYC2022008), by the Agricultural Science and Technology Innovation Program of China (ASTIP-TRIC04, ASTIP-TRIC06). Publisher Copyright: © 2023 Elsevier Ltd

PY - 2023/6

Y1 - 2023/6

N2 - A combined chemical-bacterial process was developed to convert vegetable straw waste to high value antifungal iturins. Straws from three widely cultivated vegetable (cucumber, tomato and pepper) were evaluated as feedstocks for iturin production. Microwave assisted hydrolysis with very dilute acid (0.2% w/w H2SO4) achieved efficient reducing sugar recovery. The high glucose concentration in non-detoxified hydrolysate from pepper straw facilitated the optimal growth of Bacillus amyloliquefaciens strain Cas02 and stimulated the production of iturin. The fermentation parameters were optimised to enhance the iturin production efficiency. The obtained fermentation extract was further purified using macroporous adsorption resin, resulting in an iturin-rich extract that exhibited strong antifungal activity against Alternaria alternata with an IC50 of 176.44 μg/mL. Each iturin homologue was identified using NMR. Overall, 1.58 g iturin-rich extract containing 164.06 mg/g iturins was obtained from 100 g pepper straw, illustrating the great potential of valorising pepper straw via this process.

AB - A combined chemical-bacterial process was developed to convert vegetable straw waste to high value antifungal iturins. Straws from three widely cultivated vegetable (cucumber, tomato and pepper) were evaluated as feedstocks for iturin production. Microwave assisted hydrolysis with very dilute acid (0.2% w/w H2SO4) achieved efficient reducing sugar recovery. The high glucose concentration in non-detoxified hydrolysate from pepper straw facilitated the optimal growth of Bacillus amyloliquefaciens strain Cas02 and stimulated the production of iturin. The fermentation parameters were optimised to enhance the iturin production efficiency. The obtained fermentation extract was further purified using macroporous adsorption resin, resulting in an iturin-rich extract that exhibited strong antifungal activity against Alternaria alternata with an IC50 of 176.44 μg/mL. Each iturin homologue was identified using NMR. Overall, 1.58 g iturin-rich extract containing 164.06 mg/g iturins was obtained from 100 g pepper straw, illustrating the great potential of valorising pepper straw via this process.

KW - B. amyloliquefaciens

KW - Fermentation

KW - Hydrolysis

KW - Iturin

KW - Pepper straw

UR - http://www.scopus.com/inward/record.url?scp=85151515927&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2023.129010

DO - 10.1016/j.biortech.2023.129010

M3 - Article

C2 - 37011842

AN - SCOPUS:85151515927

SN - 0960-8524

VL - 378

JO - BIORESOURCE TECHNOLOGY

JF - BIORESOURCE TECHNOLOGY

M1 - 129010

ER -