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From the same journal

Promoter trapping of a novel medium-chain acyl-CoA oxidase, which is induced transcriptionally during Arabidopsis seed germination

Research output: Contribution to journalArticle

Author(s)

  • P J Eastmond
  • M A Hooks
  • D Williams
  • P Lange
  • N Bechtold
  • C Sarrobert
  • L Nussaume
  • I A Graham

Department/unit(s)

Publication details

JournalJournal of Biological Chemistry
DatePublished - 3 Nov 2000
Issue number44
Volume275
Number of pages7
Pages (from-to)34375-34381
Original languageEnglish

Abstract

The first step of peroxisomal fatty acid beta -oxidation is catalyzed by a family of acyl-CoA oxidase isozymes with distinct fatty acyl-CoA chain-length specificities. Here we identify a new acyl-CoA oxidase gene from Arabidopsis (AtACX3) following the isolation of a promoter-trapped mutant in which beta -glucuronidase expression was initially detected in the root meristem. In acx3 mutant seedlings medium-chain acyl-CoA oxidase activity was reduced by 95%, whereas long- and short-chain activities were unchanged. Despite this reduction in activity lipid catabolism and seedling development were not perturbed. AtACX3 was cloned and expressed in Escherichia coli. The recombinant enzyme displayed medium-chain acyl-CoA substrate specificity. Analysis of beta -glucuronidase activity in acx3 revealed that, in addition to constitutive expression in the root axis, AtACX3 is also up-regulated strongly in the hypocotyl and cotyledons of germinating seedlings. This suggests that beta -oxidation is regulated predominantly at the level of transcription in germinating oilseeds. After the discovery of AtACX3, the Arabidopsis acyl-CoA oxidase gene family now comprises four isozymes with substrate specificities that encompass the full range of acyl-CoA chain lengths that exist in vivo.

    Research areas

  • CARRIER PROTEIN THIOESTERASE, PEROXISOMAL BETA-OXIDATION, MALATE SYNTHASE GENE, FATTY-ACIDS, DEVELOPMENTAL REGULATION, EXPRESSION, PLANTS, RECEPTOR, BIOSYNTHESIS, PURIFICATION

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