Proof that Burkholderia strains form effective symbioses with legumes: a study of novel mimosa-nodulating strains from South America

W M Chen; S M de Faria; R Straliotto; R M Pitard; J L Simoes-Araujo; J F Chou; Y J Chou; E Barrios; A R Prescott; G N Elliott; J I Sprent; J P W Young; E K James

doi:10.1125/AEM.71.11.7461-7471.2005

Proof that Burkholderia strains form effective symbioses with legumes: a study of novel mimosa-nodulating strains from South America

W M Chen, S M de Faria, R Straliotto, R M Pitard, J L Simoes-Araujo, J F Chou, Y J Chou, E Barrios, A R Prescott, G N Elliott, J I Sprent, J P W Young, E K James

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Twenty Mimosa-nodulating bacterial strains from Brazil and Venezuela, together with eight reference Mimosa-nodulating rhizobial strains and two other beta-rhizobial strains, were examined by amplified rRNA gene restriction analysis. They fell into 16 patterns and formed a single cluster together with the known P-rhizobia, Burkholderia caribensis, Burkholderia phymatum, and Burkholderia tuberum. The 16S rRNA gene sequences of 15 of the 20 strains were determined, and all were shown to belong to the genus Burkholderia; four distinct clusters could be discerned, with strains isolated from the same host species usually clustering very closely. Five of the strains (MAP3-5, Br3407, Br3454, Br3461, and Br3469) were selected for further studies of the symbiosis-related genes nodA, the NodD-dependent regulatory consensus sequences (nod box), and nifH. The nodA and nifH sequences were very close to each other and to those of B. phymatum STM815, B. caribensis TJ182, and Cupriavidus taiwanensis LMG19424 but were relatively distant from those of B. tuberum STM678. In addition to nodulating their original hosts, all five strains could also nodulate other Mimosa spp., and all produced nodules on Mimosa pudica that had nitrogenase (acetylene reduction) activities and structures typical of effective N-2-fixing symbioses. Finally, both wild-type and green fluorescent protein-expressing transconjugant strains of Br3461 and MAP3-5 produced N-2-fixing nodules on their original hosts, Mimosa bimucronata (Br3461) and Mimosa pigra (MAP3-5), and hence this confirms strongly that Burkholderia strains can form effective symbioses with legumes.

Original language	English
Pages (from-to)	7461-7471
Number of pages	11
Journal	Applied and Environmental Microbiology
Volume	71
Issue number	11
DOIs	https://doi.org/10.1125/AEM.71.11.7461-7471.2005
Publication status	Published - Nov 2005

Keywords

FAST-GROWING RHIZOBIA
NITROGEN-FIXATION
RALSTONIA-TAIWANENSIS
TROPICAL LEGUMES
ROOT-NODULES
TREE LEGUMES
SP NOV.
LEGUMINOSAE
PLANTS
BRAZIL

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10.1125/AEM.71.11.7461-7471.2005

Cite this

Chen, W. M., de Faria, S. M., Straliotto, R., Pitard, R. M., Simoes-Araujo, J. L., Chou, J. F., Chou, Y. J., Barrios, E., Prescott, A. R., Elliott, G. N., Sprent, J. I., Young, J. P. W., & James, E. K. (2005). Proof that Burkholderia strains form effective symbioses with legumes: a study of novel mimosa-nodulating strains from South America. Applied and Environmental Microbiology, 71(11), 7461-7471. https://doi.org/10.1125/AEM.71.11.7461-7471.2005

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title = "Proof that Burkholderia strains form effective symbioses with legumes: a study of novel mimosa-nodulating strains from South America",

abstract = "Twenty Mimosa-nodulating bacterial strains from Brazil and Venezuela, together with eight reference Mimosa-nodulating rhizobial strains and two other beta-rhizobial strains, were examined by amplified rRNA gene restriction analysis. They fell into 16 patterns and formed a single cluster together with the known P-rhizobia, Burkholderia caribensis, Burkholderia phymatum, and Burkholderia tuberum. The 16S rRNA gene sequences of 15 of the 20 strains were determined, and all were shown to belong to the genus Burkholderia; four distinct clusters could be discerned, with strains isolated from the same host species usually clustering very closely. Five of the strains (MAP3-5, Br3407, Br3454, Br3461, and Br3469) were selected for further studies of the symbiosis-related genes nodA, the NodD-dependent regulatory consensus sequences (nod box), and nifH. The nodA and nifH sequences were very close to each other and to those of B. phymatum STM815, B. caribensis TJ182, and Cupriavidus taiwanensis LMG19424 but were relatively distant from those of B. tuberum STM678. In addition to nodulating their original hosts, all five strains could also nodulate other Mimosa spp., and all produced nodules on Mimosa pudica that had nitrogenase (acetylene reduction) activities and structures typical of effective N-2-fixing symbioses. Finally, both wild-type and green fluorescent protein-expressing transconjugant strains of Br3461 and MAP3-5 produced N-2-fixing nodules on their original hosts, Mimosa bimucronata (Br3461) and Mimosa pigra (MAP3-5), and hence this confirms strongly that Burkholderia strains can form effective symbioses with legumes.",

keywords = "FAST-GROWING RHIZOBIA, NITROGEN-FIXATION, RALSTONIA-TAIWANENSIS, TROPICAL LEGUMES, ROOT-NODULES, TREE LEGUMES, SP NOV., LEGUMINOSAE, PLANTS, BRAZIL",

author = "Chen, {W M} and {de Faria}, {S M} and R Straliotto and Pitard, {R M} and Simoes-Araujo, {J L} and Chou, {J F} and Chou, {Y J} and E Barrios and Prescott, {A R} and Elliott, {G N} and Sprent, {J I} and Young, {J P W} and James, {E K}",

year = "2005",

month = nov,

doi = "10.1125/AEM.71.11.7461-7471.2005",

language = "English",

volume = "71",

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journal = "Applied and Environmental Microbiology",

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Chen, WM, de Faria, SM, Straliotto, R, Pitard, RM, Simoes-Araujo, JL, Chou, JF, Chou, YJ, Barrios, E, Prescott, AR, Elliott, GN, Sprent, JI, Young, JPW & James, EK 2005, 'Proof that Burkholderia strains form effective symbioses with legumes: a study of novel mimosa-nodulating strains from South America', Applied and Environmental Microbiology, vol. 71, no. 11, pp. 7461-7471. https://doi.org/10.1125/AEM.71.11.7461-7471.2005

TY - JOUR

T1 - Proof that Burkholderia strains form effective symbioses with legumes: a study of novel mimosa-nodulating strains from South America

AU - Chen, W M

AU - de Faria, S M

AU - Straliotto, R

AU - Pitard, R M

AU - Simoes-Araujo, J L

AU - Chou, J F

AU - Chou, Y J

AU - Barrios, E

AU - Prescott, A R

AU - Elliott, G N

AU - Sprent, J I

AU - Young, J P W

AU - James, E K

PY - 2005/11

Y1 - 2005/11

N2 - Twenty Mimosa-nodulating bacterial strains from Brazil and Venezuela, together with eight reference Mimosa-nodulating rhizobial strains and two other beta-rhizobial strains, were examined by amplified rRNA gene restriction analysis. They fell into 16 patterns and formed a single cluster together with the known P-rhizobia, Burkholderia caribensis, Burkholderia phymatum, and Burkholderia tuberum. The 16S rRNA gene sequences of 15 of the 20 strains were determined, and all were shown to belong to the genus Burkholderia; four distinct clusters could be discerned, with strains isolated from the same host species usually clustering very closely. Five of the strains (MAP3-5, Br3407, Br3454, Br3461, and Br3469) were selected for further studies of the symbiosis-related genes nodA, the NodD-dependent regulatory consensus sequences (nod box), and nifH. The nodA and nifH sequences were very close to each other and to those of B. phymatum STM815, B. caribensis TJ182, and Cupriavidus taiwanensis LMG19424 but were relatively distant from those of B. tuberum STM678. In addition to nodulating their original hosts, all five strains could also nodulate other Mimosa spp., and all produced nodules on Mimosa pudica that had nitrogenase (acetylene reduction) activities and structures typical of effective N-2-fixing symbioses. Finally, both wild-type and green fluorescent protein-expressing transconjugant strains of Br3461 and MAP3-5 produced N-2-fixing nodules on their original hosts, Mimosa bimucronata (Br3461) and Mimosa pigra (MAP3-5), and hence this confirms strongly that Burkholderia strains can form effective symbioses with legumes.

AB - Twenty Mimosa-nodulating bacterial strains from Brazil and Venezuela, together with eight reference Mimosa-nodulating rhizobial strains and two other beta-rhizobial strains, were examined by amplified rRNA gene restriction analysis. They fell into 16 patterns and formed a single cluster together with the known P-rhizobia, Burkholderia caribensis, Burkholderia phymatum, and Burkholderia tuberum. The 16S rRNA gene sequences of 15 of the 20 strains were determined, and all were shown to belong to the genus Burkholderia; four distinct clusters could be discerned, with strains isolated from the same host species usually clustering very closely. Five of the strains (MAP3-5, Br3407, Br3454, Br3461, and Br3469) were selected for further studies of the symbiosis-related genes nodA, the NodD-dependent regulatory consensus sequences (nod box), and nifH. The nodA and nifH sequences were very close to each other and to those of B. phymatum STM815, B. caribensis TJ182, and Cupriavidus taiwanensis LMG19424 but were relatively distant from those of B. tuberum STM678. In addition to nodulating their original hosts, all five strains could also nodulate other Mimosa spp., and all produced nodules on Mimosa pudica that had nitrogenase (acetylene reduction) activities and structures typical of effective N-2-fixing symbioses. Finally, both wild-type and green fluorescent protein-expressing transconjugant strains of Br3461 and MAP3-5 produced N-2-fixing nodules on their original hosts, Mimosa bimucronata (Br3461) and Mimosa pigra (MAP3-5), and hence this confirms strongly that Burkholderia strains can form effective symbioses with legumes.

KW - FAST-GROWING RHIZOBIA

KW - NITROGEN-FIXATION

KW - RALSTONIA-TAIWANENSIS

KW - TROPICAL LEGUMES

KW - ROOT-NODULES

KW - TREE LEGUMES

KW - SP NOV.

KW - LEGUMINOSAE

KW - PLANTS

KW - BRAZIL

U2 - 10.1125/AEM.71.11.7461-7471.2005

DO - 10.1125/AEM.71.11.7461-7471.2005

M3 - Article

SN - 0099-2240

VL - 71

SP - 7461

EP - 7471

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 11

ER -