Protein engineering 20 years on

Research output: Contribution to journalLiterature reviewpeer-review

Abstract

It is 20 years since site-directed mutagenesis was first used to modify the active site of an enzyme of known structure and mechanism. Since then, this method has contributed far-reaching insights into catalysis, specificity, stability and folding of proteins. Engineered proteins are now being used in industry and for the improved treatment of human disease.

Original languageEnglish
Pages (from-to)964-970
Number of pages8
JournalNature reviews. Molecular cell biology
Volume3
Issue number12
DOIs
Publication statusPublished - Dec 2002

Keywords

  • SITE-DIRECTED MUTAGENESIS
  • TRANSFER-RNA-SYNTHETASE
  • LACTATE-DEHYDROGENASE FRAMEWORK
  • TRANSITION-STATE
  • IN-VITRO
  • SUBSTRATE-SPECIFICITY
  • MOLECULAR EVOLUTION
  • BLOOD SUBSTITUTES
  • ESCHERICHIA-COLI
  • ENZYME CATALYSIS

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