Protein engineering 20 years on

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From the same journal

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Luzio, J. P., Pryor, P. R. & Bright, N. A., Aug 2007
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Review article in Nature reviews. Molecular cell biology
RNA polymerases I and III, growth control and cancer.
White, R. J., 2005
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Research output: Contribution to journal › Literature review › peer-review

Published copy (DOI)

10.1038/nrm975

Author(s)

Department/unit(s)

Chemistry

Publication details

Journal	Nature reviews. Molecular cell biology
Date	Published - Dec 2002
Issue number	12
Volume	3
Number of pages	8
Pages (from-to)	964-970
Original language	English

Abstract

It is 20 years since site-directed mutagenesis was first used to modify the active site of an enzyme of known structure and mechanism. Since then, this method has contributed far-reaching insights into catalysis, specificity, stability and folding of proteins. Engineered proteins are now being used in industry and for the improved treatment of human disease.

Research areas

SITE-DIRECTED MUTAGENESIS, TRANSFER-RNA-SYNTHETASE, LACTATE-DEHYDROGENASE FRAMEWORK, TRANSITION-STATE, IN-VITRO, SUBSTRATE-SPECIFICITY, MOLECULAR EVOLUTION, BLOOD SUBSTITUTES, ESCHERICHIA-COLI, ENZYME CATALYSIS

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