Proteomic analysis of Leishmania mexicana differentiation

Philip G Nugent, Saiful A Karsani, Robin Wait, Jane Tempero, Deborah F Smith

Research output: Contribution to journalArticlepeer-review


We have resolved the proteome of axenically differentiated Leishmania mexicana parasites by two-dimensional gel electrophoresis (2DE), employing optimised, robust and reproducible procedures, and visualised (by silver staining) approximately 2000 protein species in each of three developmental stages: procyclic promastigotes, metacyclic promastigotes and amastigotes. This analysis has used homogeneous populations of these parasite stages, characterised according to their morphology, protease and nuclease activity profiles and expression of stage-specific antigens. Following comparison of the whole proteome profiles between stages, 47 spots were found to be stage-specific, while a further 100 spots changed in intensity during differentiation. The majority of "unique" spots were expressed during the infective stages of parasite differentiation, metacyclic promastigotes and amastigotes. CapLC-QTOF mass spectrometry has allowed the identification of 47 protein species to date, including a number which are only detected in the amastigote stage. Proteins identified are members of eight functionally related groupings, some of which are implicated in infectivity and host-parasite interactions.

Original languageEnglish
Pages (from-to)51-62
Number of pages12
Issue number1
Publication statusPublished - Jul 2004


  • Animals
  • Electrophoresis, Gel, Two-Dimensional
  • Gene Expression Regulation
  • Leishmania mexicana
  • Life Cycle Stages
  • Mass Spectrometry
  • Proteome
  • Protozoan Proteins
  • Silver Staining

Cite this