Proteomic evidence of dietary sources in ancient dental calculus

TY - JOUR

T1 - Proteomic evidence of dietary sources in ancient dental calculus

AU - Hendy, Jessica

AU - Warinner, Christina

AU - Bouwman, Abigail

AU - Collins, Matthew J

AU - Fiddyment, Sarah

AU - Fischer, Roman

AU - Hagan, Richard

AU - Hofman, Courtney A

AU - Holst, Malin

AU - Chaves, Eros

AU - Klaus, Lauren

AU - Larson, Greger

AU - Mackie, Meaghan

AU - McGrath, Krista

AU - Mundorff, Amy Z

AU - Radini, Anita

AU - Rao, Huiyun

AU - Trachsel, Christian

AU - Velsko, Irina M

AU - Speller, Camilla F

N1 - © 2018 The Authors.

PY - 2018/7/25

Y1 - 2018/7/25

N2 - Archaeological dental calculus has emerged as a rich source of ancient biomolecules, including proteins. Previous analyses of proteins extracted from ancient dental calculus revealed the presence of the dietary milk protein β-lactoglobulin, providing direct evidence of dairy consumption in the archaeological record. However, the potential for calculus to preserve other food-related proteins has not yet been systematically explored. Here we analyse shotgun metaproteomic data from 100 archaeological dental calculus samples ranging from the Iron Age to the post-medieval period (eighth century BC to nineteenth century AD) in England, as well as 14 dental calculus samples from contemporary dental patients and recently deceased individuals, to characterize the range and extent of dietary proteins preserved in dental calculus. In addition to milk proteins, we detect proteomic evidence of foodstuffs such as cereals and plant products, as well as the digestive enzyme salivary amylase. We discuss the importance of optimized protein extraction methods, data analysis approaches and authentication strategies in the identification of dietary proteins from archaeological dental calculus. This study demonstrates that proteomic approaches can robustly identify foodstuffs in the archaeological record that are typically under-represented due to their poor macroscopic preservation.

AB - Archaeological dental calculus has emerged as a rich source of ancient biomolecules, including proteins. Previous analyses of proteins extracted from ancient dental calculus revealed the presence of the dietary milk protein β-lactoglobulin, providing direct evidence of dairy consumption in the archaeological record. However, the potential for calculus to preserve other food-related proteins has not yet been systematically explored. Here we analyse shotgun metaproteomic data from 100 archaeological dental calculus samples ranging from the Iron Age to the post-medieval period (eighth century BC to nineteenth century AD) in England, as well as 14 dental calculus samples from contemporary dental patients and recently deceased individuals, to characterize the range and extent of dietary proteins preserved in dental calculus. In addition to milk proteins, we detect proteomic evidence of foodstuffs such as cereals and plant products, as well as the digestive enzyme salivary amylase. We discuss the importance of optimized protein extraction methods, data analysis approaches and authentication strategies in the identification of dietary proteins from archaeological dental calculus. This study demonstrates that proteomic approaches can robustly identify foodstuffs in the archaeological record that are typically under-represented due to their poor macroscopic preservation.

KW - Archaeology

KW - DNA, Ancient/analysis

KW - Dental Calculus/chemistry

KW - Diet/history

KW - England

KW - History, 15th Century

KW - History, 16th Century

KW - History, 17th Century

KW - History, 18th Century

KW - History, 19th Century

KW - History, Ancient

KW - History, Medieval

KW - Proteome

KW - Dietary reconstruction

KW - Dental calculus

KW - Proteomics

KW - Mass spectrometry

UR - http://www.scopus.com/inward/record.url?scp=85051569797&partnerID=8YFLogxK

U2 - 10.1098/rspb.2018.0977

DO - 10.1098/rspb.2018.0977

M3 - Article

C2 - 30051838

SN - 0962-8452

VL - 285

JO - Proceedings of the Royal Society B: Biological Sciences

JF - Proceedings of the Royal Society B: Biological Sciences

IS - 1883

M1 - 20180977

ER -