Ptychography--a label free, high-contrast imaging technique for live cells using quantitative phase information

TY - JOUR

T1 - Ptychography--a label free, high-contrast imaging technique for live cells using quantitative phase information

AU - Marrison, Joanne

AU - Räty, Lotta

AU - Marriott, Poppy

AU - O'Toole, Peter

PY - 2013/8/6

Y1 - 2013/8/6

N2 - Cell imaging often relies on synthetic or genetic fluorescent labels, to provide contrast which can be far from ideal for imaging cells in their in vivo state. We report on the biological application of a, label-free, high contrast microscopy technique known as ptychography, in which the image producing step is transferred from the microscope lens to a high-speed phase retrieval algorithm. We demonstrate that this technology is appropriate for label-free imaging of adherent cells and is particularly suitable for reporting cellular changes such as mitosis, apoptosis and cell differentiation. The high contrast, artefact-free, focus-free information rich images allow dividing cells to be distinguished from non-dividing cells by a greater than two-fold increase in cell contrast, and we demonstrate this technique is suitable for downstream automated cell segmentation and analysis.

AB - Cell imaging often relies on synthetic or genetic fluorescent labels, to provide contrast which can be far from ideal for imaging cells in their in vivo state. We report on the biological application of a, label-free, high contrast microscopy technique known as ptychography, in which the image producing step is transferred from the microscope lens to a high-speed phase retrieval algorithm. We demonstrate that this technology is appropriate for label-free imaging of adherent cells and is particularly suitable for reporting cellular changes such as mitosis, apoptosis and cell differentiation. The high contrast, artefact-free, focus-free information rich images allow dividing cells to be distinguished from non-dividing cells by a greater than two-fold increase in cell contrast, and we demonstrate this technique is suitable for downstream automated cell segmentation and analysis.

KW - Animals

KW - Cells, Cultured

KW - Equipment Design

KW - Equipment Failure Analysis

KW - Humans

KW - Image Enhancement

KW - Lighting

KW - Microscopy, Phase-Contrast

KW - Reproducibility of Results

KW - Sensitivity and Specificity

U2 - 10.1038/srep02369

DO - 10.1038/srep02369

M3 - Article

C2 - 23917865

SN - 2045-2322

VL - 3

JO - Scientific Reports

JF - Scientific Reports

M1 - 2369

ER -