Pulmonary dendritic cells and alveolar macrophages are regulated by gamma delta T cells during the resolution of S. pneumoniae-induced inflammation

TY - JOUR

T1 - Pulmonary dendritic cells and alveolar macrophages are regulated by gamma delta T cells during the resolution of S. pneumoniae-induced inflammation

AU - Kirby, A. C.

AU - Newton, D. J.

AU - Carding, S. R.

AU - Kaye, P. M.

N1 - Copyright (c) 2007 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

PY - 2007/5

Y1 - 2007/5

N2 - gamma delta T cells commonly associate with mucosal and epithelial sites, fulfilling a variety of immunoregulatory functions. While lung gamma delta T cells have well-characterized proinflammatory activity, their potential role in the resolution of lung inflammation has yet to be explored in any detail. Indeed, given the importance of minimizing inflammation, the cellular mechanisms driving the resolution of lung inflammation are poorly understood. Using a murine model of acute Streptococcus pneumoniae-mediated lung inflammation, we now show that resolution of inflammation following bacterial clearance is associated with a > 30-fold increase in gamma delta T-cell number. Although inflammation eventually resolves in TCR delta(-/-) mice, elevated numbers of alveolar macrophages and pulmonary dendritic cells, and the appearance of well-formed granulomas in lungs of TCR delta(-/-) mice, together indicated a role for gamma delta T cells in regulating mononuclear phagocyte number. Ex vivo, both alveolar macrophages and pulmonary dendritic cells were susceptible to lung gamma delta T cell-mediated cytotoxicity, the first demonstration of such activity against a dendritic cell population. These findings support a model whereby expansion of gamma delta T cells helps restore mononuclear phagocyte numbers to homeostatic levels, protecting the lung from the consequences of inappropriate inflammation. Copyright (c) 2007 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

AB - gamma delta T cells commonly associate with mucosal and epithelial sites, fulfilling a variety of immunoregulatory functions. While lung gamma delta T cells have well-characterized proinflammatory activity, their potential role in the resolution of lung inflammation has yet to be explored in any detail. Indeed, given the importance of minimizing inflammation, the cellular mechanisms driving the resolution of lung inflammation are poorly understood. Using a murine model of acute Streptococcus pneumoniae-mediated lung inflammation, we now show that resolution of inflammation following bacterial clearance is associated with a > 30-fold increase in gamma delta T-cell number. Although inflammation eventually resolves in TCR delta(-/-) mice, elevated numbers of alveolar macrophages and pulmonary dendritic cells, and the appearance of well-formed granulomas in lungs of TCR delta(-/-) mice, together indicated a role for gamma delta T cells in regulating mononuclear phagocyte number. Ex vivo, both alveolar macrophages and pulmonary dendritic cells were susceptible to lung gamma delta T cell-mediated cytotoxicity, the first demonstration of such activity against a dendritic cell population. These findings support a model whereby expansion of gamma delta T cells helps restore mononuclear phagocyte numbers to homeostatic levels, protecting the lung from the consequences of inappropriate inflammation. Copyright (c) 2007 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

KW - gamma delta T cells

KW - dendritic cells

KW - alveolar macrophages

KW - inflammation

KW - resolution

KW - MURINE PNEUMOCOCCAL PNEUMONIA

KW - ACTIVATED MACROPHAGES

KW - INFLUENZA PNEUMONIA

KW - INFECTIOUS-DISEASE

KW - LYMPHOCYTES

KW - LUNG

KW - MICE

KW - CONTRIBUTES

KW - NEUTROPHILS

KW - CLEARANCE

U2 - 10.1002/path.2149

DO - 10.1002/path.2149

M3 - Article

C2 - 17370296

SN - 0022-3417

VL - 212

SP - 29

EP - 37

JO - Journal of Pathology

JF - Journal of Pathology

IS - 1

ER -