PURIFICATION, CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF THE VANADIUM-DEPENDENT HALOPEROXIDASE FROM CORALLINA-OFFICINALIS

C  RUSH; A  WILLETTS; G  DAVIES; Z  DAUTER; H  WATSON; J  LITTLECHILD

PURIFICATION, CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF THE VANADIUM-DEPENDENT HALOPEROXIDASE FROM CORALLINA-OFFICINALIS

C RUSH, A WILLETTS, G DAVIES, Z DAUTER, H WATSON, J LITTLECHILD

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

The vanadium-dependent haloperoxidase from the seaweed Corallina officinalis has been purified to homogeneity and crystallised, The protein is reported to be a hexamer of 12 x 64,000 Da, contains no haem, and is dependent on vanadium for activity. The crystals are grown from polyethylene glycol (PEG) 6,000 and 0.4 M potassium chloride, They are stable and diffract to better than 2 A resolution, They are of a cubic space group I23 (or I2(1)3) with cell dimensions a = b = c = 310 Angstrom.

Original language	English
Pages (from-to)	244-246
Number of pages	3
Journal	FEBS Letters
Volume	359
Issue number	2-3
Publication status	Published - 13 Feb 1995

Keywords

HALOPEROXIDASE
VANADIUM ENZYME
CRYSTAL
X-RAY DIFFRACTION
BIOTRANSFORMATION
ASCOPHYLLUM-NODOSUM
REACTION-MECHANISM
BROMOPEROXIDASE
CHLOROPEROXIDASE
PILULIFERA
PEROXIDASE
ENZYME

Cite this

@article{819baa9ca78647c9a9ddbe64157d5c9d,

title = "PURIFICATION, CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF THE VANADIUM-DEPENDENT HALOPEROXIDASE FROM CORALLINA-OFFICINALIS",

abstract = "The vanadium-dependent haloperoxidase from the seaweed Corallina officinalis has been purified to homogeneity and crystallised, The protein is reported to be a hexamer of 12 x 64,000 Da, contains no haem, and is dependent on vanadium for activity. The crystals are grown from polyethylene glycol (PEG) 6,000 and 0.4 M potassium chloride, They are stable and diffract to better than 2 A resolution, They are of a cubic space group I23 (or I2(1)3) with cell dimensions a = b = c = 310 Angstrom.",

keywords = "HALOPEROXIDASE, VANADIUM ENZYME, CRYSTAL, X-RAY DIFFRACTION, BIOTRANSFORMATION, ASCOPHYLLUM-NODOSUM, REACTION-MECHANISM, BROMOPEROXIDASE, CHLOROPEROXIDASE, PILULIFERA, PEROXIDASE, ENZYME",

author = "C RUSH and A WILLETTS and G DAVIES and Z DAUTER and H WATSON and J LITTLECHILD",

year = "1995",

month = feb,

day = "13",

language = "English",

volume = "359",

pages = "244--246",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley-Blackwell",

number = "2-3",

}

TY - JOUR

T1 - PURIFICATION, CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF THE VANADIUM-DEPENDENT HALOPEROXIDASE FROM CORALLINA-OFFICINALIS

AU - RUSH, C

AU - WILLETTS, A

AU - DAVIES, G

AU - DAUTER, Z

AU - WATSON, H

AU - LITTLECHILD, J

PY - 1995/2/13

Y1 - 1995/2/13

N2 - The vanadium-dependent haloperoxidase from the seaweed Corallina officinalis has been purified to homogeneity and crystallised, The protein is reported to be a hexamer of 12 x 64,000 Da, contains no haem, and is dependent on vanadium for activity. The crystals are grown from polyethylene glycol (PEG) 6,000 and 0.4 M potassium chloride, They are stable and diffract to better than 2 A resolution, They are of a cubic space group I23 (or I2(1)3) with cell dimensions a = b = c = 310 Angstrom.

AB - The vanadium-dependent haloperoxidase from the seaweed Corallina officinalis has been purified to homogeneity and crystallised, The protein is reported to be a hexamer of 12 x 64,000 Da, contains no haem, and is dependent on vanadium for activity. The crystals are grown from polyethylene glycol (PEG) 6,000 and 0.4 M potassium chloride, They are stable and diffract to better than 2 A resolution, They are of a cubic space group I23 (or I2(1)3) with cell dimensions a = b = c = 310 Angstrom.

KW - HALOPEROXIDASE

KW - VANADIUM ENZYME

KW - CRYSTAL

KW - X-RAY DIFFRACTION

KW - BIOTRANSFORMATION

KW - ASCOPHYLLUM-NODOSUM

KW - REACTION-MECHANISM

KW - BROMOPEROXIDASE

KW - CHLOROPEROXIDASE

KW - PILULIFERA

KW - PEROXIDASE

KW - ENZYME

M3 - Article

SN - 0014-5793

VL - 359

SP - 244

EP - 246

JO - FEBS Letters

JF - FEBS Letters

IS - 2-3

ER -