Quantification of an arbuscular mycorrhizal fungus, Glomus mosseae, within plant roots by competitive polymerase chain reaction

S G Edwards; A H Fitter; J P W Young

doi:10.1017/S0953756297004218

Quantification of an arbuscular mycorrhizal fungus, Glomus mosseae, within plant roots by competitive polymerase chain reaction

S G Edwards, A H Fitter, J P W Young

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

An assay based on the competitive polymerase chain reaction (PCR) was developed to quantify Glomus mosseae, an arbuscular mycorrhizal (AM) fungus, within plant roots. Using previously designed G. mosseae specific primers, a heterologous internal standard was constructed by amplifying Pseudomonas DNA under low stringency annealing conditions. Go-amplification of G. mosseae and internal standard DNA within leek root extracts provided accurate quantification of target DNA. Colonization of leek roots by G. mosseae was monitored in a comparative study by competitive PCR and microscopy, a conventional method of quantification. These two methods gave closely parallel data for G. mosseae colonization from three different inoculum levels over a 6 week period Results indicate that competitive PCR is a sensitive and accurate method of quantification. The major advantage of competitive PCR over microscopy is that it can quantify specific AM fungi.

Original language	English
Pages (from-to)	1440-1444
Number of pages	5
Journal	Mycological Research
Volume	101
Issue number	12
DOIs	https://doi.org/10.1017/S0953756297004218
Publication status	Published - Dec 1997

Keywords

COLONIZING ROOTS
PCR
DNA
IDENTIFICATION
QUANTITATION
GENERATION
DIVERSITY
GLOMALES
PRIMERS

Access to Document

10.1017/S0953756297004218

http://dx.doi.org/10.1017/S0953756297004218

Cite this

@article{b3bcd237e9474be0a56835b2b8f32e57,

title = "Quantification of an arbuscular mycorrhizal fungus, Glomus mosseae, within plant roots by competitive polymerase chain reaction",

abstract = "An assay based on the competitive polymerase chain reaction (PCR) was developed to quantify Glomus mosseae, an arbuscular mycorrhizal (AM) fungus, within plant roots. Using previously designed G. mosseae specific primers, a heterologous internal standard was constructed by amplifying Pseudomonas DNA under low stringency annealing conditions. Go-amplification of G. mosseae and internal standard DNA within leek root extracts provided accurate quantification of target DNA. Colonization of leek roots by G. mosseae was monitored in a comparative study by competitive PCR and microscopy, a conventional method of quantification. These two methods gave closely parallel data for G. mosseae colonization from three different inoculum levels over a 6 week period Results indicate that competitive PCR is a sensitive and accurate method of quantification. The major advantage of competitive PCR over microscopy is that it can quantify specific AM fungi.",

keywords = "COLONIZING ROOTS, PCR, DNA, IDENTIFICATION, QUANTITATION, GENERATION, DIVERSITY, GLOMALES, PRIMERS",

author = "Edwards, {S G} and Fitter, {A H} and Young, {J P W}",

year = "1997",

month = dec,

doi = "10.1017/S0953756297004218",

language = "English",

volume = "101",

pages = "1440--1444",

journal = "Mycological Research",

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TY - JOUR

T1 - Quantification of an arbuscular mycorrhizal fungus, Glomus mosseae, within plant roots by competitive polymerase chain reaction

AU - Edwards, S G

AU - Fitter, A H

AU - Young, J P W

PY - 1997/12

Y1 - 1997/12

N2 - An assay based on the competitive polymerase chain reaction (PCR) was developed to quantify Glomus mosseae, an arbuscular mycorrhizal (AM) fungus, within plant roots. Using previously designed G. mosseae specific primers, a heterologous internal standard was constructed by amplifying Pseudomonas DNA under low stringency annealing conditions. Go-amplification of G. mosseae and internal standard DNA within leek root extracts provided accurate quantification of target DNA. Colonization of leek roots by G. mosseae was monitored in a comparative study by competitive PCR and microscopy, a conventional method of quantification. These two methods gave closely parallel data for G. mosseae colonization from three different inoculum levels over a 6 week period Results indicate that competitive PCR is a sensitive and accurate method of quantification. The major advantage of competitive PCR over microscopy is that it can quantify specific AM fungi.

AB - An assay based on the competitive polymerase chain reaction (PCR) was developed to quantify Glomus mosseae, an arbuscular mycorrhizal (AM) fungus, within plant roots. Using previously designed G. mosseae specific primers, a heterologous internal standard was constructed by amplifying Pseudomonas DNA under low stringency annealing conditions. Go-amplification of G. mosseae and internal standard DNA within leek root extracts provided accurate quantification of target DNA. Colonization of leek roots by G. mosseae was monitored in a comparative study by competitive PCR and microscopy, a conventional method of quantification. These two methods gave closely parallel data for G. mosseae colonization from three different inoculum levels over a 6 week period Results indicate that competitive PCR is a sensitive and accurate method of quantification. The major advantage of competitive PCR over microscopy is that it can quantify specific AM fungi.

KW - COLONIZING ROOTS

KW - PCR

KW - DNA

KW - IDENTIFICATION

KW - QUANTITATION

KW - GENERATION

KW - DIVERSITY

KW - GLOMALES

KW - PRIMERS

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DO - 10.1017/S0953756297004218

M3 - Article

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VL - 101

SP - 1440

EP - 1444

JO - Mycological Research

JF - Mycological Research

IS - 12

ER -