Quantifying Caspase-1 Activity in Murine Macrophages

Dave Boucher, Amy Chan, Connie Ross, Kate Schroder

Research output: Contribution to journalArticlepeer-review


The caspase-1 protease is a core component of multiprotein inflammasome complexes, which play a critical role in regulating the secretion of mature, bioactive pro-inflammatory cytokines interleukin (IL)-1β and IL-18. The activity of caspase-1 is often measured indirectly, by monitoring cleavage of cellular caspase-1 substrates, processing of caspase-1 itself, or by quantifying cell death. Here we describe methods for eliciting caspase-1 activity in murine macrophages, via activation of the NLRP3, NAIP/NLRC4 or AIM2 inflammasomes. We then describe a simple fluorogenic assay for directly quantifying cellular caspase-1 activity.

Original languageEnglish
Pages (from-to)163-176
Number of pages14
JournalMethods in molecular biology (Clifton, N.J.)
Publication statusPublished - 11 Jan 2018


  • Animals
  • Apoptosis Regulatory Proteins/metabolism
  • Bone Marrow/enzymology
  • Caspase 1/metabolism
  • Cytokines/metabolism
  • Inflammasomes/immunology
  • Macrophages/enzymology
  • Mice

Cite this