Quantifying Caspase-1 Activity in Murine Macrophages

Dave Boucher; Amy Chan; Connie Ross; Kate Schroder

doi:10.1007/978-1-4939-7568-6_14

Quantifying Caspase-1 Activity in Murine Macrophages

Dave Boucher, Amy Chan, Connie Ross, Kate Schroder

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

The caspase-1 protease is a core component of multiprotein inflammasome complexes, which play a critical role in regulating the secretion of mature, bioactive pro-inflammatory cytokines interleukin (IL)-1β and IL-18. The activity of caspase-1 is often measured indirectly, by monitoring cleavage of cellular caspase-1 substrates, processing of caspase-1 itself, or by quantifying cell death. Here we describe methods for eliciting caspase-1 activity in murine macrophages, via activation of the NLRP3, NAIP/NLRC4 or AIM2 inflammasomes. We then describe a simple fluorogenic assay for directly quantifying cellular caspase-1 activity.

Original language	English
Pages (from-to)	163-176
Number of pages	14
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	1725
DOIs	https://doi.org/10.1007/978-1-4939-7568-6_14
Publication status	Published - 11 Jan 2018

Keywords

Animals
Apoptosis Regulatory Proteins/metabolism
Bone Marrow/enzymology
Caspase 1/metabolism
Cytokines/metabolism
Inflammasomes/immunology
Macrophages/enzymology
Mice

Access to Document

10.1007/978-1-4939-7568-6_14

https://espace.library.uq.edu.au/view/UQ:3ebe196

Cite this

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title = "Quantifying Caspase-1 Activity in Murine Macrophages",

abstract = "The caspase-1 protease is a core component of multiprotein inflammasome complexes, which play a critical role in regulating the secretion of mature, bioactive pro-inflammatory cytokines interleukin (IL)-1β and IL-18. The activity of caspase-1 is often measured indirectly, by monitoring cleavage of cellular caspase-1 substrates, processing of caspase-1 itself, or by quantifying cell death. Here we describe methods for eliciting caspase-1 activity in murine macrophages, via activation of the NLRP3, NAIP/NLRC4 or AIM2 inflammasomes. We then describe a simple fluorogenic assay for directly quantifying cellular caspase-1 activity.",

keywords = "Animals, Apoptosis Regulatory Proteins/metabolism, Bone Marrow/enzymology, Caspase 1/metabolism, Cytokines/metabolism, Inflammasomes/immunology, Macrophages/enzymology, Mice",

author = "Dave Boucher and Amy Chan and Connie Ross and Kate Schroder",

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doi = "10.1007/978-1-4939-7568-6_14",

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AU - Boucher, Dave

AU - Chan, Amy

AU - Ross, Connie

AU - Schroder, Kate

PY - 2018/1/11

Y1 - 2018/1/11

N2 - The caspase-1 protease is a core component of multiprotein inflammasome complexes, which play a critical role in regulating the secretion of mature, bioactive pro-inflammatory cytokines interleukin (IL)-1β and IL-18. The activity of caspase-1 is often measured indirectly, by monitoring cleavage of cellular caspase-1 substrates, processing of caspase-1 itself, or by quantifying cell death. Here we describe methods for eliciting caspase-1 activity in murine macrophages, via activation of the NLRP3, NAIP/NLRC4 or AIM2 inflammasomes. We then describe a simple fluorogenic assay for directly quantifying cellular caspase-1 activity.

AB - The caspase-1 protease is a core component of multiprotein inflammasome complexes, which play a critical role in regulating the secretion of mature, bioactive pro-inflammatory cytokines interleukin (IL)-1β and IL-18. The activity of caspase-1 is often measured indirectly, by monitoring cleavage of cellular caspase-1 substrates, processing of caspase-1 itself, or by quantifying cell death. Here we describe methods for eliciting caspase-1 activity in murine macrophages, via activation of the NLRP3, NAIP/NLRC4 or AIM2 inflammasomes. We then describe a simple fluorogenic assay for directly quantifying cellular caspase-1 activity.

KW - Animals

KW - Apoptosis Regulatory Proteins/metabolism

KW - Bone Marrow/enzymology

KW - Caspase 1/metabolism

KW - Cytokines/metabolism

KW - Inflammasomes/immunology

KW - Macrophages/enzymology

KW - Mice

U2 - 10.1007/978-1-4939-7568-6_14

DO - 10.1007/978-1-4939-7568-6_14

M3 - Article

C2 - 29322417

SN - 1940-6029

VL - 1725

SP - 163

EP - 176

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

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